Rat IL-2(Interleukin-2) Introduction

Interleukin-2 (IL-2) is an interleukin, a type of cytokine signaling molecule in the immune system. It is a 15.5–16 kDa protein that regulates the activities of white blood cells (leukocytes, often lymphocytes) that are responsible for immunity. IL-2 is part of the body's natural response to microbial infection, and in discriminating between foreign ("non-self") and "self". IL-2 mediates its effects by binding to IL-2 receptors, which are expressed by lymphocytes. The major sources of IL-2 are activated CD4+ T cells and activated CD8+ T cells.

Rat IL-2(Interleukin-2) ELISA KIT Test Method

This kit uses sandwich ELISA to detect the concentration of Rat IL-2.

Rat IL-2-specific monoclonal antibody has been pre-coated in the wells of the supplied microplate. Standards samples and controls are added to interact with the immobilized antibody. A sandwich complex is formed by additional anti-Mouse IL-2 antibody with HRP-Streptavidin. TMB solution is added to react with the sandwich forming optical signal measured by microplate reader. The concentration of Bovine IL-2 in the sample can be calculated by comparing the absorbance of the sample with the standard curve.

Reference:

1. GRCh38: Ensembl release 89: ENSG00000109471-Ensembl, May 2017
2. GRCm38: Ensembl release 89: ENSMUSG00000027720-Ensembl, May 2017
3. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
4. "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
5. Arenas-Ramirez N, Woytschak J, Boyman O (December 2015). "Interleukin-2: Biology, Design and Application" (PDF). Trends in Immunology. 36 (12): 763–777. doi:10.1016/j.it .2015.10.003. PMID 26572555.