Rat IL-4(Interleukin-4) Introduciton

IL4 is involved in at least several B cell activation processes and other cell types. It is a costimulator of DNA synthesis. It induces the expression of MHC class II molecules on resting B cells. It enhances the secretion and cell surface expression of IgE and IgG1. It also regulates the low-affinity Fc receptor expression of IgE (CD23) on lymphocytes and monocytes. Genetic variation of IL4 may be a cause of susceptibility to ischemic stroke (ISCHSTR); also known as cerebrovascular accident or cerebral infarction.Besides its proinflammatory effects, IL-23 promotes angiogenesis. 

Rat IL-4(Interleukin-4) ELISA Kit Test method

This kit uses sandwich ELISA to detect the concentration of Rat IL-4.

Rat IL-4-specific monoclonal antibody has been pre-coated in the wells of the supplied microplate. Standards samples and controls are added to interact with the immobilized antibody. A sandwich complex is formed by additional anti-Rat IL-4 antibody with HRP-Streptavidin. TMB solution is added to react with the sandwich for ming optical signal measured by microplate reader. The concentration of Rat IL-4 in the sample can be calculated by comparing the absorbance of the sample with the standard curve.

Reference:

1."Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.

2.Gadani, Sachin P.; Cronk, James C.; Norris, Geoffrey T.; Kipnis, Jonathan (2012-11-01). "Interleukin-4: A Cytokine to Remember". Journal of Immunology. 189 (9): 4213 –4219. doi:10.4049/jimmunol.1202246. ISSN 0022-1767. PMC 3481177. PMID 23087426.

3.Sandova V, Pavlasova GM, Seda V, Cerna KA, Sharma S, Palusova V, Brychtova Y, Pospisilova S, Fernandes SM, Panovska A, Doubek M, Davids MS, Brown JR, Mayer J, Mraz M (July 1, 2021) . "IL4-STAT6 signaling induces CD20 in chronic lymphocytic leukemia and this axis is repressed by PI3Kδ inhibitor idelalisib". Haematologica. X (X): X. doi:10.3324/haematol.2021.278644. PMID 34196167.