PTGS2/COX-2(Prostaglandin G/H synthase 2) Introduction 

Cyclooxygenase (COX), officially known as prostaglandin-endoperoxide synthase (PTGS), is an enzyme (specifically, a family of isozymes, EC 1.14.99.1) that is responsible for formation of prostanoids, including thromboxane and prostaglandins such as prostacyclin, from arachidonic acid. A member of the animal-type heme peroxidase family, it is also known as prostaglandin G/H synthase. The specific reaction catalyzed is the conversion from arachidonic acid to Prostaglandin H2, via a short-living Prostaglandin G2 intermediate. Inhibition of COX-2 using celecoxib has been shown to reduce the immunosuppressive TGFβ expression in hepatocytes attenuating EMT in human hepatocellular carcinoma. 

Rat PTGS2/COX-2(Prostaglandin G/H synthase 2) ELISA Kit Test method

This kit uses sandwich ELISA to detect the concentration of  PTGS2/COX-2(Prostaglandin G/H synthase 2). The microtiter plate provided in this kit has been pre-coated with an antibody specific to PTGS2/COX-2(Prostaglandin G/H synthase 2). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to PTGS2/COX-2(Prostaglandin G/H synthase 2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain PTGS2/COX-2(Prostaglandin G/H synthase 2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of PTGS2/COX-2(Prostaglandin G/H synthase 2) in the samples is then determined by comparing the OD of the samples to the standard curve.

Reference:

1,  PDB: 1CQE​; Picot D, Loll PJ, Garavito RM (January 1994). "The X-ray crystal structure of the membrane protein prostaglandin H2 synthase-1". Nature. 367 (6460): 243–9. Bibcode:1994Natur.367..243P. doi:10.1038/367243a0. PMID 8121489. S2CID 4340064.

2, PDB: 6COX​; Kurumbail RG, Stevens AM, Gierse JK, McDonald JJ, Stegeman RA, Pak JY, Gildehaus D, Miyashiro JM, Penning TD, Seibert K, Isakson PC, Stallings WC (1996). "Structural basis for selective inhibition of cyclooxygenase-2 by anti-inflammatory agents". Nature. 384 (6610): 644–8. Bibcode:1996Natur.384..644K. doi:10.1038/384644a0. PMID 8967954. S2CID 4326310.