S100B(S100 Calcium Binding Protein B) Introduction

S100 calcium-binding protein B (S100B) is a protein of the S-100 protein family. S100 proteins are localized in the cytoplasm and nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21; however, this gene is located at 21q22.3. S100B is glial-specific and is expressed primarily by astrocytes, but not all astrocytes express S100B. It has been shown that S100B is only expressed by a subtype of mature astrocytes that ensheath blood vessels and by NG2-expressing cells.  This protein may function in neurite extension, proliferation of melanoma cells, stimulation of Ca2+ fluxes, inhibition of PKC-mediated phosphorylation, astrocytosis and axonal proliferation, and inhibition of microtubule assembly. In the developing CNS it acts as a neurotrophic factor and neuronal survival protein. In the adult organism it is usually elevated due to nervous system damage, which makes it a potential clinical marker.

Rat S100B(S100 Calcium Binding Protein B) ELISA Kit Test method

This kit uses sandwich ELISA to detect the concentration of  S100B(S100 Calcium Binding Protein B) The microtiter plate provided in this kit has been pre-coated with an antibody specific to S100B(S100 Calcium Binding Protein B). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to S100B(S100 Calcium Binding Protein B). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain of S100B, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of S100B(S100 Calcium Binding Protein B) in the samples is then determined by comparing the OD of the samples to the standard curve.

Reference:

1, "Salmonella infection data for S100b". Wellcome Trust Sanger Institute.

2."Citrobacter infection data for S100b". Wellcome Trust Sanger Institute.