Human Anti-mullerian hormone | 617-EKU02459

Species Reactivity

Human

Sample Type

Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Detection Range

185.2-15,000pg/mL

Sensitivity

64.9pg/mL

Estimated Turnaround Time

7-11 business days

Target Name

AMH / Anti-Mullerian Hormone

Target Synonyms

MIF; MIH; MIS; Müllerian Inhibiting Factor; Müllerian Inhibiting Hormone; Müllerian Inhibiting Substance

Precision

Intra-Assay: CV<10%, Inter-Assay: CV<12%

Detection Method

Competitive Inhibition

Assay Time

2 hours

Application

ELISA

Assay Type

Competitive Inhibition

Shipping Condition

Ice packs

Storage

Short term: 4°C; Long term: see manual.

Precaution of Use

The Stop Solution is acidic. Do not allow to contact skin or eyes.

UniProt ID

P03971

Specificity

This assay has high sensitivity and excellent specificity for detection of Anti-Mullerian Hormone (AMH). No significant cross-reactivity or interference between Anti-Mullerian Hormone (AMH) and analogues was observed.

Test principle

The microplate provided in this kit has been pre-coated with an antibody specific to Interferon Alpha (IFNa). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Interferon Alpha (IFNa). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Interferon Alpha (IFNa) level in the sample or standard.;

Assay procedure summary

1. Prepare all reagents, samples and standards
2. Add 50µl standard or sample to each well.And then add 50µl prepared Detection Reagent A immediately.Shake and mix. Incubate 1 hour at 37°C
3. Aspirate and wash 3 times
4. Add 100µl prepared Detection Reagent B. Incubate 30 minutes at 37°C
5. Aspirate and wash 5 times
6. Add 90µl Substrate Solution. Incubate 10-20 minutes at 37°C
7. Add 50µl Stop Solution. Read at 450 nm immediately.

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Restrictions

For Research Use Only. Not for use in diagnostic procedures. Not for human or animal drug or food use.

Quality Systems

The kit is manufactured at ISO 9001 and ISO 13485 certified facilities.

Quality Assurance

Biomatik ELISA Kits are not typically pre-assembled as finished products due to limited shelf life. Before shipping, each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Detection Range, Sensitivity, and Precision. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation. Please always refer to the hard copy manual included in the kit before experiment.

Research Area

Cytokine;Infection immunity