FineTest
Human Anti-MPV IgM(Anti-Monkeypox Virus IgM) ELISA Kit | EH4997
- SKU:
- EH4997
- Weight:
- 0 KGS
- Shipping:
- Calculated at Checkout
Description
Specificity
This assay has high sensitivity and excellent specificity for detection of Anti-MPV IgM. No significant cross-reactivity or interference between Anti-MPV IgM and analogues was observed.
Note: Limited by current skills and knowledge, it is difficult for us to complete the cross-reactivity detection between Anti-MPV IgM and all the analogues, therefore, cross reaction may still exist.
Recovery
Matrices listed below were spiked with certain level of Anti-MPV IgM and the recovery rates were calculated by comparing the measured value to the expected amount of Anti-MPV IgM in samples.
Matrix | Recovery Range (%) | Average (%) |
Serum(n=5) | 89-99 | 95 |
EDTA Plasma(n=5) | 90-99 | 93 |
Heparin Plasma(n=5) | 92-102 | 97 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Anti-MPV IgM and their serial dilutions. The results were demonstrated by percentage of calculated concentration to the expectation.
Sample | 1:2 | 1:4 | 1:8 |
Serum(n=5) | 87-103% | 85-101% | 86-95% |
EDTA Plasma(n=5) | 83-100% | 84-95% | 86-97% |
Heparin Plasma(n=5) | 80-99% | 80-99% | 82-96% |
Precision
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Stability
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 10% within the expiration date under appropriate storage condition.
Standard(n=5) | 37°C for 1 month | 2-8°C for 6 months |
Average (%) | 80 | 95-100 |
To minimize extra influence on performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is strongly suggested that the same operator performs the whole assay from the beginning to the end.
Additional Information
Size: |
96T |
Reactivity: |
Human |
Range: |
1.563-100ng/ml |
Sensitivity: |
0.938ng/ml |
Application: |
For quantitative detection of Anti-MPV IgM in serum, plasma and other biological fluids. |
Storage: |
2-8°C |
Principle: |
Indirect |