SIRT1(Sirtuin 1) Introduciton

Immunoglobulin A (IgA, also referred to as sIgA in its secretory form) is an antibody that plays a crucial role in the immune function of mucous membranes. The amount of IgA produced in association with mucosal membranes is greater than all other types of antibody combined. In absolute terms, between three and five grams are secreted into the intestinal lumen each day. This represents up to 15% of total immunoglobulins produced throughout the body. In the blood, IgA interacts with an Fc receptor called FcαRI (or CD89), which is expressed on immune effector cells, to initiate inflammatory reactions. Ligation of FcαRI by IgA containing immune complexes causes antibody-dependent cell-mediated cytotoxicity (ADCC), degranulation of eosinophils and basophils, phagocytosis by monocytes, macrophages, and neutrophils, and triggering of respiratory burst activity by polymorphonuclear leukocytes

Human SIRT1(Sirtuin 1) ELISA Kit Test method

This kit uses sandwich ELISA to detect the concentration of  SIRT1 The microtiter plate provided in this kit has been pre-coated with an antibody specific to SIRT1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to SIRT1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain of SIRT1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of SIRT1 in the samples is then determined by comparing the OD of the samples to the standard curve.

Reference:

1, Fagarasan S, Honjo T (January 2003). "Intestinal IgA synthesis: regulation of front-line body defences". Nature Reviews. Immunology. 3 (1): 63–72. doi:10.1038/nri982. PMID 12511876. S2CID 2586305.

2, Brandtzaeg P, Pabst R (November 2004). "Let's go mucosal: communication on slippery ground". Trends in Immunology. 25 (11): 570–7. doi:10.1016/j.it.2004.09.005. PMID 15489184.

3, Macpherson AJ, Slack E (November 2007). "The functional interactions of commensal bacteria with intestinal secretory IgA". Current Opinion in Gastroenterology. 23 (6): 673–8. doi:10.1097/MOG.0b013e3282f0d012. PMID 17906446. S2CID 8445606.