SCF Antibody

SCF Antibody | 5165 | ProSci

Host: Rabbit

Reactivity: Human, Mouse, Rat

Homology: Predicted species reactivity based on immunogen sequence: Bovine: (71%)

Immunogen: Anti-SCF antibody (5165) was raised against a peptide corresponding to 18 amino acids near the center of human SCF.
The immunogen is located within amino acids 100-150 of SCF.

Research Area: Stem Cell

Tested Application: E, WB, IHC-P, IF

Application: WB: 1-2 μg/mL; IHC: 2.5 μg/mL; IF: 20 μg/mL.
Antibody validated: Western Blot in human, mouse and rat samples; Immunohistochemistry in mouse samples; Immunofluorescence in human samples. All other applications and species not yet tested.

Specificiy: N/A

Positive Control 1: Cat. No. 1202 – A431 Cell Lysate

Positive Control 2: Cat. No. 10-301 - Human Brain Tissue Slide

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: Predicted: 31kD
Observed: 36 kD (31kD + 4 N-linked Glycosylations)

Validation:

Recombinant Protein Test (Figure 2) : Anti-SCF antibodies (5165) detected human SCF recombinant protein at different concentrations.

Regulated expression validation (Figure 7 & 8) : SCF expression detected by anti-SCF antibodies (5165) was up-regulated by angiotensin II (Ang II) treatment and was down-regulated by two inhibitors (Figure 7) or CNP treatment (Figure 8) .

Isoform: Human SCF has 3 isoforms, including isoform 1 (273aa, 30.9kD) , isoform 2 (245aa, 27.9kD) and isoform 3 (238aa, 26.7kD) . Mouse SCF has 2 isoforms, including isoform 1 (273aa, 30.6kD) and isoform 2 (245aa, 27.6kD) . Rat SCF also has 2 isoforms, including isoform 1 (273aa, 30.7kD) and isoform 2 (245aa, 27.7kD) . 5165 can detect human and possibly detect mouse and rat.

Purification: SCF Antibody is affinity chromatography purified via peptide column.

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: SCF Antibody is supplied in PBS containing 0.02% sodium azide.

Concentration: 1 mg/mL

Storage Condition: SCF antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Alternate Name: SCF Antibody: SF, MGF, SCF, FPH2, KL-1, Kitl, SHEP7, Kit ligand, Mast cell growth factor

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: SCF Antibody: Stem cell factor (SCF) is the ligand of the c-Kit oncogene and is expressed by various structural and inflammatory cells in the airways. Binding of SCF by the c-Kit receptor leads to homodimerization of the receptor and the activation of signalling pathways such as PI-3, PLC-gamma, Jak/STAT, and MAP kinase pathways. SCF expression leads to the induction of mast cell survival and the expression and release of histamine, pro-inflammatory cytokines and chemokines. The inhibition of the SCF/c-Kit pathway leads to a decrease in histamine levels, mast cell and eosinophil infiltration, IL-4 production and airway hyperresponsiveness, suggesting this pathway may be a useful therapeutic target in inflammatory diseases such as asthma. At least two isoforms of SCF are known to exist.