Omalizumab ELISA (XOLAIR)

This Omalizumab ELISA is for determination of Omalizumab in human serum, plasma and cell culture supernatant. Omalizumab is a recombinant, humanized, monoclonal antibody that acts as an inhibitor for human immunoglobulin E (IgE). Omalizumab limits allergic responses in the patient, and is used in the treatment severe allergic asthma and chronic idiopathic urticarial.The method employs the quantitative sandwich enzyme immunoassay technique. Antibodies to Omalizumab are pre-coated onto microwells. Samples and standards are pipetted into microwells and human Omalizumab present in the sample are bound by the capture antibody. Then, a HRP (horseradish peroxidase) conjugated anti-Omalizumab antibody is pipetted and incubated. After washing microwells in order to remove any non-specific binding, the ready to use substrate solution (TMB) is added to microwells and color develops proportionally to the amount of Omalizumab in the sample. Color development is then stopped by addition of stop solution. Absorbance is measured at 450 nm.