Plant Preservation Mixture (PPMTM)

broad-spectrum biocide formulated for use in plant tissue culture

1. Plant Preservation Mixture (PPM) is a robust broad-spectrum biocide formulated for use in plant tissue culture.
2. PPMTM targets bacteria and fungi in plant tissue culture growth media as well as contaminated tissue.
3. It affects key enzymes in the Krebs cycle and in the Electron Transport Chain.
4. Depending on the dose and the level of contamination, PPM is a biocidal component in plant culture medium.
5. In addition, it may also function as a biostatic compound as a preventative measure.
6. When diluted with plant growth media it is effective as a microbiocide (i.e. bactericide and fungicide) against non-human health pathogenic organisms component of liquid or semi-solid plant culture media.
   
7. PPMTM is effective for most seed baring plants - angiosperm, as well as gymnosperm,- however, it is not recommended for use in ferns, mosses, algae and aquatic plants. Optimization may be required to maximize potency.
8. While PPM is an excellent tool in the prevention and elimination of culture contamination it is not a substitute for asceptic laboratory techniques and appropriate air handling systems are recommended.
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What is Plant Preservative Mixture (PPM) ?

Plant Preservative Mixture (PPM), is a broad-spectrum biocide/ for plant tissue culture. PPM™ is the ultimate solution to the never-ending struggle against microbial airborne, waterborne and endogenous contamination.

ABOUT PLANT PRESERVATIVE MIXTURE (PPM)

PPM™ is a heat stable preservative/biocide which can be used to effectively prevent or reduces microbial contamination in plant tissue culture.

At optimum doses, PPM™, which stands for Plant Preservative Mixture™, is an extremely effective Preservative/Biocide, yet does not impair in vitro seed germination, callus proliferation and callus regeneration.
PPM™ prevents the germination of both bacteria and fungi spores. It is heat stable and therefore can be autoclaved with the media.

PPM™ can be, and should be used as a standard ingredient in plant tissue culture media, and is also substantially less expensive than commonly used antibiotics.

While PPM™ was principally designed to inhibit airborne contamination, waterborne contamination and contamination introduced from human contact, it can also -- in many cases -- be used to reduce endogenous contamination.

Despite the most stringent use of sterile techniques, the contamination of plant cell and plant tissue cultures remain a persistent problem that can result in losses ranging from small number of cultures to the loss of whole batches.