Elabscience
QuicKey Pro Human IL-6 (Interleukin 6) ELISA Kit
(No reviews yet)
Write a Review
- SKU:
- E-OSEL-H0001
- Weight:
- 0 KGS
- Shipping:
- Calculated at Checkout
Description
QuicKey Pro Series
Get more sensitive and precise results with saving at least 1-2h comparing to traditional ELISA Kits. The new developed technology in house will help to accelerate your science research in a more efficient way.
Product Details
Properties
| Assay type | Sandwich-ELISA |
| Format | 96T/48T/24 |
| Assay time | 1.5h |
| Detection range | 3.13-200 pg/mL |
| Sensitivity | 1.02 pg/mL |
| Sample type &Sample volume | serum and plasma; 50μL |
| Specificity | This kit recognizes IL-6 in samples. No significant cross-reactivity or interference between IL-6 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are < 10%. |
| Application | This ELISA kit applies to the in vitro quantitative determination of IL-6 concentrations in serum and plasma. |
Test Principle
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human IL-6. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Human IL-6 are added to the micro ELISA plate wells. Human IL-6 in samples (or standards) combines with the coated antibody and HRP linked detection antibody specific to IL-6. Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450±2 nm. The concentration of Human IL-6 in the samples is then determined by comparing the OD of the samples to the standard curve.
Kit components & Storage
An unopened kit can be stored at 2-8℃ for 6 months. After test, the unused wells and reagents should be stored according to the table.
| Item | Specifications | Storage |
|---|---|---|
| Micro ELISA Plate(Dismountable) | 96T: 8 wells ×12 strips 48T: 8 wells ×6 strips 24T: 8 wells ×3 strips 96T*5: 5 plates, 96T |
2-8℃, 1 months |
| Reference Standard | 96T: 2 vials 48T/24T: 1 vial 96T*5: 10 vials |
2-8℃, use the reconstituted standard within 24h |
| Concentrated HRP Linked Detection Ab(100×) | 96T: 1 vial, 60 μL 48T/24T: 1 vial, 30 μL 96T*5: 5 vials, 60 μL |
2-8℃(Protect from light) |
| Reference Standard & Sample Diluent | 96T/48T/24T: 1 vial, 20 mL 96T*5: 5 vials, 20 mL |
2-8℃ |
| HRP Linked Ab Diluent | 96T/48T/24T: 1 vial, 14 mL 96T*5: 5 vials, 14 mL |
|
| Concentrated Wash Buffer(25×) | 96T/48T/24T: 1 vial, 30 mL 96T*5: 5 vials, 30 mL |
|
| Substrate Reagent | 96T/48T/24T: 1 vial, 10 mL 96T*5: 5 vials, 10 mL |
2-8℃(Protect from light) |
| Stop Solution | 96T/48T/24T: 1 vial, 10 mL 96T*5: 5 vials, 10 mL |
2-8℃ |
| Plate Sealer | 96T/48T/24T: 5 pieces 96T*5: 25 pieces |
|
| Product Description | 1 copy | |
| Certificate of Analysis | 1 copy |
Technical Data
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level IL-6 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level IL-6 were tested on 3 different plates, 20 replicates in each plate.
| Intra-assay Precision | Inter-assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (pg/mL) | 9.10 | 22.29 | 90.92 | 9.12 | 21.98 | 90.28 |
| Standard deviation | 0.57 | 1.13 | 3.32 | 0.53 | 1.31 | 3.25 |
| CV (%) | 6.24 | 5.06 | 3.65 | 5.84 | 5.98 | 3.60 |
Recovery
The recovery of IL-6 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.
| Sample Type | Range (%) | Average Recovery (%) |
|---|---|---|
| Serum(n=8) | 89-102 | 96 |
| EDTA plasma(n=8) | 92-105 | 97 |
Additional Information
Reactivity: |
Human |
Detection Range: |
3.13~200 pg/mL |
Sensitivity: |
1.02 pg/mL |
Related Products
QuicKey Pro Human IL-8 (Interleukin 8) ELISA Kit
Elabscience
€295.00 - €776.00
QuicKey Pro Series
Get more sensitive and precise results with saving at least 1-2h comparing to traditional ELISA Kits. The new developed technology in house will help to accelerate your science...
Human IL-6(Interleukin 6) ELISA Kit
Feiyuebio
€587.20
Feiyue’s Human IL-6 is an ELISA reagent for detection of IL-6 in human serum, plasma or cell with sensitivity, specificity and consistency.
Human IL-6(Interleukin 6) Introduciton
Interleukin-6,...
Human Interleukin 6, IL-6 ELISA KIT | CSB-E04638h
Cusabio Biotech
€350.33 - €3,279.59
Human Interleukin 6, IL-6 ELISA KIT | CSB-E04638h | Cusabio
Human Interleukin 6, IL-6 ELISA KIT is Available at Gentaur Genprice with the fastest delivery.
Online Order Payment is possible or...
Rat IL-6(Interleukin-6) ELISA Kit
Feiyuebio
€565.33
Rat IL-6(Interleukin-6) ELISA Kit Basic Information
Product Name
Rat IL-6(Interleukin-6) ELISA Kit
Catalog NO.
FY-ER3995
Alias
Rabbit IL-6(Interleukin 6) ELISA Kit
Feiyuebio
€780.09
Rabbit IL-6(Interleukin 6) Introduciton
Interleukin-6, abbreviated as interleukin-6, was originally discovered by the team of Japanese immunologist Chuzo Kishimoto. It is an interleukin that mediates...
Human Interleukin 6 receptor (IL-6R) ELISA Kit | CSB-E16524h
Cusabio Biotech
€540.45 - €4,913.85
Human Interleukin 6 receptor (IL-6R) ELISA Kit | CSB-E16524h | Cusabio
Human Interleukin 6 receptor (IL-6R) ELISA Kit is Available at Gentaur Genprice with the fastest delivery.
Online Order...
QuicKey Pro Human Cortisol ELISA Kit
Elabscience
€295.00 - €776.00
QuicKey Pro Series
Get more sensitive and precise results with saving at least 1-2h comparing to traditional ELISA Kits. The new developed technology in house will help to accelerate your science...
Mouse Interleukin 6, IL-6 ELISA KIT | CSB-E04639m
Cusabio Biotech
€350.33
Mouse Interleukin 6, IL-6 ELISA KIT | CSB-E04639m | Cusabio
Mouse Interleukin 6, IL-6 ELISA KIT is Available at Gentaur Genprice with the fastest delivery.
Online Order Payment is possible or...