QuicKey Pro Human D2D (D-Dimer) ELISA Kit

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human D2D. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Human D2D are added to the micro ELISA plate wells. Human D2D in samples (or standards) combines with the coated antibody and HRP linked detection antibody specific to D2D. Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450±2 nm. The concentration of Human D2D in the samples is then determined by comparing the OD of the samples to the standard curve.

An unopened kit can be stored at 2-8℃ for 6 months. After test, the unused wells and reagents should be stored according to the table.

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level D2D were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level D2D were tested on 3 different plates, 20 replicates in each plate.