Elabscience

QuicKey Pro Rabbit Pg (Progesterone) ELISA Kit

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E-OSEL-RB0003
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  • QuicKey Pro Rabbit Pg(Progesterone) ELISA Kit
  • QuicKey Pro Rabbit Pg(Progesterone) ELISA Kit
  • QuicKey Pro Rabbit Pg(Progesterone) ELISA Kit
  • QuicKey Pro Rabbit Pg(Progesterone) ELISA Kit
€295.00 - €776.00

Description

QuicKey Pro Series

Get more sensitive and precise results with saving at least 1-2h comparing to traditional ELISA Kits. The new developed technology in house will help to accelerate your science research in a more efficient way.

Product Details

Properties

Assay type Competitive-ELISA
Format 96T/48T/24
Assay time 1.5h
Detection range 0.16-10 ng/mL
Sensitivity 0.05 ng/mL
Sample type &Sample volume serum and plasma; 50μL
Specificity This kit recognizes Pg in samples. No significant cross-reactivity or interference between Pg and analogues was observed.
Reproducibility Both intra-CV and inter-CV are < 10%.
Application This ELISA kit applies to the in vitro quantitative determination of Pg concentrations in serum and plasma.

Test Principle

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Rabbit Pg. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Rabbit Pg are added to the micro ELISA plate wells. Rabbit Pg in samples (or standards) competes with a fixed amount of Pg on the solid phase supporter for sites on the HRP linked detection antibody specific to Pg. Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450±2 nm. The concentration of Rabbit Pg in the samples is then determined by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 6 months. After test, the unused wells and reagents should be stored according to the table.
Item Specifications Storage
Micro ELISA Plate(Dismountable) 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips
24T: 8 wells ×3 strips
96T*5: 5 plates, 96T		 2-8℃, 1 months
Reference Standard 96T: 2 vials
48T/24T: 1 vial
96T*5: 10 vials		 2-8℃, use the reconstituted standard within 24h
Concentrated HRP Linked Detection Ab(100×) 96T: 1 vial, 60 μL
48T/24T: 1 vial, 30 μL
96T*5: 5 vials, 60 μL		 2-8℃(Protect from light)
Reference Standard & Sample Diluent 96T/48T/24T: 1 vial, 20 mL
96T*5: 5 vials, 20 mL		 2-8℃
HRP Linked Ab Diluent 96T/48T/24T: 1 vial, 14 mL
96T*5: 5 vials, 14 mL		  
Concentrated Wash Buffer(25×) 96T/48T/24T: 1 vial, 30 mL
96T*5: 5 vials, 30 mL
Substrate Reagent 96T/48T/24T: 1 vial, 10 mL
96T*5: 5 vials, 10 mL		 2-8℃(Protect from light)
Stop Solution 96T/48T/24T: 1 vial, 10 mL
96T*5: 5 vials, 10 mL		 2-8℃
Plate Sealer 96T/48T/24T: 5 pieces
96T*5: 25 pieces		  
Product Description 1 copy
Certificate of Analysis 1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Pg were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Pg were tested on 3 different plates, 20 replicates in each plate.

  Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (ng/mL) 0.47 1.53 4.35 0.42 1.60 4.70
Standard deviation 0.03 0.06 0.19 0.02 0.07 0.23
CV (%) 5.35 4.10 4.39 5.45 4.18 4.88

Recovery

The recovery of Pg spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 89-104 95
EDTA plasma(n=8) 94-109 101

Linearity

Samples were spiked with high concentrations of Rabbit Pg and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Synonyms Progesterone，Pg
Research Area Signal Transduction
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Additional Information

Reactivity:
Rabbit
Detection Range:
0.16~10 ng/mL
Sensitivity:
0.05 ng/mL
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