Elabscience
Total Glutathione (T-GSH)/Oxidized Glutathione (GSSG) Colorimetric Assay Kit
- SKU:
- E-BC-K097-M
- Weight:
- 0 KGS
- Shipping:
- Calculated at Checkout
Description
Detection principle
GSSG is reduced to GSH by glutathione reductase, and GSH can react with DTNB to produce GSSG and yellow TNB. The amount of total glutathione (GSSG+GSH) determines the amount of yellow TNB. Thus the total glutathione can be calculated by measuring the OD value at 412 nm. The content of GSSG can be determined by first removing GSH from the sample with appropriate reagent and then using the above reaction principle.
Performance characteristics
| Synonyms | T-GSH,GSSG |
| Sample type | Serum,plasma,animal tissue,red blood cells,cultured cells |
| Sensitivity | 0.36 μmol/L T-GSH |
| Detection range | 0.36-30 μmol/L T-GSH |
| Detection method | Colorimetric method |
| Assay type | Quantitative |
| Assay time | 100 min |
| Precision | Average inter-assay CV: 3.9%Average intra-assay CV: 0.6% |
| Other instruments required | Micropipettor, Centrifuge, Incubator, Vortex mixer |
| Other reagents required | PBS (0.01 M, pH 7.4), Absolute ethanol |
| Storage | -20℃ |
| Valid period | 6 months |
Dilution of sample
It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.36-30 μmol/L T-GSH).
The recommended dilution factor for different samples is as follows (for reference only):
|
Sample type |
Dilution factor |
|
Human plasma |
1 |
|
10% Rat liver tissue homogenate |
10-20 |
|
10% Rat kidney tissue homogenate |
1 |
|
10% Rat heart tissue homogenate |
10-20 |
|
10% Mouse brain tissue homogenate |
2-5 |
|
HepG2 cells |
1 |
Note: The diluent is reagent 3 working solution.
Additional Information
Detection instrument: |
Microplate reader(405-415 nm,optimum wavelength: 412 nm) |
Detection method: |
Colorimetric method |
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