Beclin-1 Antibody

Beclin-1 Antibody | 3613 | ProSci

Host: Rabbit

Reactivity: Human, Mouse, Rat

Homology: Predicted species reactivity based on immunogen sequence: Bovine: (94%) , Pig: (88%)

Immunogen: Anti-Beclin-1 antibody (3613) was raised against a peptide corresponding to 17 amino acids near the center of human Beclin-1.
The immunogen is located between 40-90 amino acids of Beclin-1.

Research Area: Apoptosis, Autophagy, Cancer

Tested Application: E, WB, IHC-P, IF

Application: WB: 1 μg/mL; IHC: 2 μg/mL; IF: 20 μg/mL.
Antibody validated: Western Blot in human, mouse and rat samples; Immunohistochemistry in human, mouse, and rat samples; Immunofluorescence in human and mouse samples. All other applications and species not yet tested.

Specificiy: N/A

Positive Control 1: Cat. No. 1210 - HEK293 Cell Lysate

Positive Control 2: Cat. No. 1403 - Mouse Brain Tissue Lysate

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: Predicted: 52 kD
Observed: 60 kD

Validation: Antibody validated: Western Blot in human samples; Immunohistochemistry in human, mouse, and rat samples and Immunofluorescence in human and mouse samples. All other applications and species not yet tested.

Isoform: Human Beclin-1 has one isoform (450aa, 52kD) . Mouse Beclin-1 has one isoform (448aa, 52kD) and Rat Beclin-1 also has one isoform (448aa, 52kD) . 3613 can detect human, mouse and rat.

Purification: Beclin-1 Antibody is affinity chromatography purified via peptide column.

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: Beclin-1 Antibody is supplied in PBS containing 0.02% sodium azide.

Concentration: 1 mg/mL

Storage Condition: Beclin-1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Alternate Name: Beclin-1 Antibody: ATG6, VPS30, beclin1, GT197, Beclin-1, Coiled-coil myosin-like BCL2-interacting protein

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: Beclin-1 Antibody: Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. Beclin-1, a coiled-coil Bcl-2-interacting protein homologous to the yeast autophagy gene apg6, is a mammalian autophagy gene that can inhibit tumorigenesis and is expressed at reduced levels in human breast carcinoma, suggesting that defects in autophagy proteins may contribute to the development or progression of tumors. Bcl-2 can bind to Beclin-1 and inhibit Beclin-1-dependent autophagy in yeast and mammalian cells, suggesting that Bcl-2 functions as an anti-autophagy protein as well as an anti-apoptotic protein, which helps maintain autophagy at levels that are more compatible with cell survival rather than cell death.