PD-1 Antibody [7A11B1]

PD-1 Antibody [7A11B1] | PM-5177 | ProSci

Host: Mouse

Reactivity: Human, Mouse, Rat

Homology: N/A

Immunogen: A ~150 amino acid recombinant protein from near the amino terminus of mouse PD-1.

Research Area: Apoptosis

Tested Application: E, WB, IHC-P, IF

Application: PD-1 antibody can be used for detection of PD-1 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in human and mouse samples and Immunofluorescence in human and mouse samples. All other applications and species not yet tested.

Specificiy: N/A

Positive Control 1: Cat. No. 95-115 - PD-1 Recombinant Protein

Positive Control 2: N/A

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: N/A

Validation: N/A

Isoform: N/A

Purification: PD-1 Monoclonal Antibody is Protein A purified.

Clonality: Monoclonal

Clone: 7A11B1

Isotype: IgG1

Conjugate: Unconjugated

Physical State: Liquid

Buffer: PD-1 Monoclonal Antibody is supplied in PBS containing 0.02% sodium azide.

Concentration: 1 mg/mL

Storage Condition: PD-1 monoclonal antibody can be stored at -20˚C, stable for one year.

Alternate Name: PD-1 Antibody [7A11B1] : PD1, PD-1, CD279, SLEB2, hPD-1, hPD-l, hSLE1

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: PD-1 Monoclonal Antibody: Cell-mediated immune responses are initiated by T lymphocytes that are themselves stimulated by cognate peptides bound to MHC molecules on antig en-presenting cells (APC) . T-cell activation is generally self-limited as activated T cells express receptors such as PD-1 (also known as PDCD-1) that mediate inhibitory signals from the APC. PD-1 can bind two different but related ligands, PDL-1 and PDL-2. Upon binding to either of these ligands, signals generated by PD-1 inhibit the activation of the immune response in the absence of "danger signals" such as LPS or other molecules associated with bacteria or other pathogens. Evidence for this is seen in PD1-null mice who exhibit hyperactivated immune systems and autoimmune diseases. Despite its predicted molecular weight, PD-1 often migrates at higher molecular weight in SDS-PAGE.