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Ethidium Bromide Staining: Principles, Protocol, and Applications in Molecular Biology Ethidium bromide staining is one of the most widely used techniques in molecular biology for the visualization of nucleic acids after gel electrophoresis. The method relies on the ability of ethidium bromide (EtBr), a fluorescent intercalating dye, to bind DNA and RNA molecules and emit strong fluorescence under ultraviolet (UV) light. Because of its high sensitivity and simplicity, this technique remains a st …