FY-EH4452 Human GHRL(Ghrelin) ELISA Kit

GHRL(Ghrelin) Basic information

Ghrelin is a hormone produced by enteroendocrine cells of the gastrointestinal tract, especially the stomach, and is often called a "hunger hormone" because it increases the drive to eat. Blood levels of ghrelin are highest before meals when hungry, returning to lower levels after mealtimes. Ghrelin may help prepare for food intake by increasing gastric motility and stimulating the secretion of gastric acid.

Human GHRL(Ghrelin) ELISA Kit test method

This kit was based on Competitive-ELISA detection method. The microtiter plate provided in this kit has been pre-coated with target. During the reaction, target in the sample or standard competes with a fixed amount of target on the solid phase supporter for sites on the Biotinylated Detection Antibody specific to target. Excess conjugate and unbound sample or standard are washed from the plate, and HRP-Streptavidin (SABC) is added to each microplate well and incubated. Then TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm. The concentration of target in the samples is then determined by comparing the OD of the samples to the standard curve. 

Reference:

1. GRCh38: Ensembl release 89: ENSG00000157017 - Ensembl, May 2017

2. GRCm38: Ensembl release 89: ENSMUSG00000064177 - Ensembl, May 2017

3. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.