Human GDF15(Growth Differentiation Factor 15) Introduction

GDF15 is a protein belonging to the transforming growth factor beta superfamily. Under normal conditions, GDF-15 is expressed in low concentrations in most organs and upregulated because of injury of organs such as liver, kidney, heart and lung. The function of GDF-15 is not fully clear but it seems to have a role in regulating inflammatory pathways and to be involved in regulating apoptosis, cell repair and cell growth, which are biological processes observed in cardiovascular and neoplastic disorders. GDF-15 has shown to be a strong prognostic protein in patients with different diseases such as heart diseases and cancer.

Human GDF15(Growth Differentiation Factor 15) ELISA Kit Test Method

This kit uses sandwich ELISA to detect the concentration of Human GDF15.

Human GDF15-specific monoclonal antibody has been pre-coated in the wells of the supplied microplate.Standards samples and controls are added to interact with the immobilized antibody. A sandwich complex is formed by additional anti-Human GDF15 antibody with HRP-Streptavidin.TMB solution is added to react with the sandwich forming optical signal measured by microplate reader.The concentration of Human GDF15 in the sample can be calculated by comparing the absorbance of the sample with the standard curve.  

Reference:

1. GRCh38: Ensembl release 89: ENSG00000130513 - Ensembl, May 2017.
2. GRCm38: Ensembl release 89: ENSMUSG00000038508 - Ensembl, May 2017.
3. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.