Human HMGB1(High mobility group protein B1) ELISA Kit Basic Information 

Feiyue's Human HMGB1 is an ELISA reagent for detection of HMGB1 antibody, plasma or cell with sensitivity, specificity and consistency.

Human HMGB1(High mobility group protein B1) Introduction

HMGB1 is a protein. HMG-1 belongs to the high mobility group and contains a HMG-box domain. Like the histones, HMGB1 is among the most important chromatin proteins. In the nucleus HMGB1 interacts with nucleosomes, transcription factors, and histones. This nuclear protein organizes the DNA and regulates transcription. After binding, HMGB1 bends DNA, which facilitates the binding of other proteins. HMGB1 supports transcription of many genes in interactions with many transcription factors. It also interacts with nucleosomes to loosen packed DNA and remodel the chromatin. Contact with core histones changes the structure of nucleosomes. The presence of HMGB1 in the nucleus depends on posttranslational modifications. 

Human HMGB1(High mobility group protein B1) ELISA Kit Test Method

This kit uses sandwich ELISA to detect the concentration of Human HMGB1.

Human HMGB1-specific monoclonal antibody has been pre-coated in the wells of the supplied microplate.Standards samples and controls are added to interact with the immobilized antibody. A sandwich complex is formed by additional anti-Human HMGB1 antibody with HRP-Streptavidin.TMB solution is added to react with the sandwich forming opticalsignal measured by microplate reader.The concentration of Human HMGB1 in the sample can be calculated by comparing the absorbance of the sample with the standard curve.