Human IL-5(Interleukin 5) Introduciton

IL-5 is a 115-amino acid (in human, 133 in the mouse) -long TH2 cytokine that is part of the hematopoietic family. Unlike other members of this cytokine family (namely interleukin 3 and GM-CSF), this glycoprotein in its active form is a homodimer. Through binding to the interleukin-5 receptor, interleukin 5 stimulates B cell growth and increases immunoglobulin secretion-primarily IgA. It is also a key mediator in eosinophil activation. Belongs to the IL-5 family.

Human IL-5(Interleukin 5)  ELISA Kit Test method

This kit uses sandwich ELISA to detect the concentration of human IL-5.

Human IL-5-specific monoclonal antibody has been pre-coated in the wells of the supplied microplate.Standards samples and controls are added to interact with the immobilized antibody. A sandwich complex is formed by additional anti-human IL-5 antibody with HRP-Streptavidin.TMB solution is added to react with the sandwich forming optical signal measured by microplate reader.The concentration of human IL-5 in the sample can be calculated by comparing the absorbance of the sample with the standard curve.  

Reference:

1.GRCh38: Ensembl release 89: ENSG00000113525-Ensembl, May 2017

2.GRCm38: Ensembl release 89: ENSMUSG00000036117-Ensembl, May 2017

3."Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.

4."Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.

5.Milburn MV, Hassell AM, Lambert MH, Jordan SR, Proudfoot AE, Graber P, Wells TN (May 1993). "A novel dimer configuration revealed by the crystal structure at 2.4 A resolution of human interleukin-5". Nature. 363 ( 6425): 172–176. doi:10.1038/363172a0. PMID 8483502. S2CID 4254991.