FY-EM4662 Mouse IL-5(Interleukin 5) ELISA Kit

IL-5(Interleukin 5) Basic information

Through binding to the interleukin-5 receptor, interleukin 5 stimulates B cell growth and increases immunoglobulin secretion - primarily IgA. It is also a key mediator in eosinophil activation.

IL-5 is a 115-amino acid (in human, 133 in the mouse) -long TH2 cytokine that is part of the hematopoietic family. Unlike other members of this cytokine family (namely interleukin 3 and GM-CSF), this glycoprotein in its active form is a homodimer.

The IL-5 gene is located on chromosome 11 in the mouse, and chromosome 5 in humans, in close proximity to the genes encoding IL-3, IL-4, and granulocyte-macrophage colony-stimulating factor (GM-CSF), which are often co-expressed in TH2 cells. Interleukin-5 is also expressed by eosinophils and has been observed in the mast cells of asthmatic airways by immunohistochemistry. IL-5 expression is regulated by several transcription factors including GATA3.

Mouse IL-5(Interleukin 5) ELISA Kit test method

Feiyue’s Mouse IL-5(Interleukin 5) is an ELISA reagent for detection of IL-5(Interleukin 5) in Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

This kit uses sandwich ELISA to detect the concentration of Mouse IL-5(Interleukin 5). Mouse IL-5(Interleukin 5)-specific monoclonal antibody has been pre-coated in the wells of the supplied microplate. Standards samples and controls are added to interact with the immobilized antibody. A sandwich complex is formed by additional anti-Mouse IL-5(Interleukin 5) antibody with HRP-Streptavidin. TMB solution is added to react with the sandwich for ming optical signal measured by microplate reader. The concentration of Mouse IL-5(Interleukin 5) in the sample can be calculated by comparing the absorbance of the sample with the standard curve.

Reference:

 1.GRCh38: Ensembl release 89: ENSG00000113525 - Ensembl, May 2017

 2.GRCm38: Ensembl release 89: ENSMUSG00000036117 - Ensembl, May 2017

 3."Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.

 4."Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.