Human LEP(Leptin) Introduciton

Leptin (from Greek λεπτός leptos, "thin") is a hormone predominantly made by adipose cells and enterocytes in the small intestine that helps to regulate energy balance by inhibiting hunger, which in turn diminishes fat storage in adipocytes. Leptin acts on cell receptors in the arcuate and ventromedial nuclei, as well as other parts of the hypothalamus and dopaminergic neurons of the ventral tegmental area, consequently mediating feeding. Although regulation of fat stores is deemed to be the primary function of leptin, it also plays a role in other physiological processes, as evidenced by its many sites of synthesis other than fat cells, and the many cell types beyond hypothalamic cells that have leptin receptors. Many of these additional functions are yet to be fully defined. 

Human LEP(Leptin) ELISA Kit Test method

This kit uses sandwich ELISA to detect the concentration of Human LEP.

Human LEP-specific monoclonal antibody has been pre-coated in the wells of the supplied microplate. Standards samples and controls are added to interact with the immobilized antibody. A sandwich complex is formed by additional anti-Human LEP antibody with HRP-Streptavidin. TMB solution is added to react with the sandwich for ming optical signal measured by microplate reader. The concentration of Human LEP in the sample can be calculated by comparing the absorbance of the sample with the standard curve.

Reference:

1. GRCh38: Ensembl release 89: ENSG00000174697 - Ensembl, May 2017
2. GRCm38: Ensembl release 89: ENSMUSG00000059201 - Ensembl, May 2017
3. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
4. "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
5. Zhang F, Basinski MB, Beals JM, Briggs SL, Churgay LM, Clawson DK, et al. (May 1997). "Crystal structure of the obese protein leptin-E100". Nature. 387 (6629): 206–9. Bibcode:1997Natur.387..206Z. doi:10.1038/387206a0. PMID 9144295. S2CID 716518.