Elabscience

Glutathione-S-Transferase (GST) Activity Assay Kit(DTNB method)

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SKU:: E-BC-K800-M
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Description
Additional Information

Description

Detection principle

Glutathione S-transferase is a kind of enzyme related to liver detoxification, which is often used as an indicator of liver injury. GST can resist the damage of endogenous and exogenous electronophilic substances, and plays an important role in the anti-tumor process.

Performance characteristics

Synonyms	GST
Sample type	Serum, plasma, animal tissue
Sensitivity	2.1 U/L
Detection range	2.1-92.8 U/L
Detection method	Colorimetric method
Assay type	Enzyme Activity
Assay time	65 min
Precision	Average inter-assay CV: 6.4%Average intra-assay CV: 1.8%
Other instruments required	Test tube, Micropipettor, Vortex mixer, Centrifuge, 37℃ incubator.
Other reagents required	PBS (0.01 M, pH 7.4)
Storage	2-8℃
Valid period	6 months

Dilution of sample

It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (2.1-92.8 U/L).

The recommended dilution factor for different samples is as follows (for reference only):

Sample type	Dilution factor
Human plasma (serum)	1
Horse serum	1
Rat serum	1
Rabbit serum	1
Porcine serum	1
10% Rat kidney tissue homogenate	1
10% Rat brain tissue homogenate	1
10% Rat liver tissue homogenate	1
10% Rat spleen tissue homogenate	1
10% Rat lung tissue homogenate	1