Elabscience
Glutathione-S-Transferase (GST) Activity Assay Kit(DTNB method)
- SKU:
- E-BC-K800-M
- Weight:
- 0 KGS
- Shipping:
- Calculated at Checkout
Description
Detection principle
Glutathione S-transferase is a kind of enzyme related to liver detoxification, which is often used as an indicator of liver injury. GST can resist the damage of endogenous and exogenous electronophilic substances, and plays an important role in the anti-tumor process.
Performance characteristics
| Synonyms | GST |
| Sample type | Serum, plasma, animal tissue |
| Sensitivity | 2.1 U/L |
| Detection range | 2.1-92.8 U/L |
| Detection method | Colorimetric method |
| Assay type | Enzyme Activity |
| Assay time | 65 min |
| Precision | Average inter-assay CV: 6.4%Average intra-assay CV: 1.8% |
| Other instruments required | Test tube, Micropipettor, Vortex mixer, Centrifuge, 37℃ incubator. |
| Other reagents required | PBS (0.01 M, pH 7.4) |
| Storage | 2-8℃ |
| Valid period | 6 months |
Dilution of sample
It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (2.1-92.8 U/L).
The recommended dilution factor for different samples is as follows (for reference only):
|
Sample type |
Dilution factor |
|
Human plasma (serum) |
1 |
|
Horse serum |
1 |
|
Rat serum |
1 |
|
Rabbit serum |
1 |
|
Porcine serum |
1 |
|
10% Rat kidney tissue homogenate |
1 |
|
10% Rat brain tissue homogenate |
1 |
|
10% Rat liver tissue homogenate |
1 |
|
10% Rat spleen tissue homogenate |
1 |
|
10% Rat lung tissue homogenate |
1 |
Note: The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4).
Additional Information
Detection instrument: |
Microplate reader(400 nm-420 nm,optimum wavelength: 412 nm) |
Detection method: |
Colorimetric method |
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