PD-1 Antibody | 4065

PD-1 Antibody | 4065 | ProSci

Host: Rabbit

Reactivity: Human, Mouse, Rat

Homology: N/A

Immunogen: Anti-PD-1 antibody (4065) was raised against a peptide corresponding to 16 amino acids near the carboxy terminus of human PD-1.
The immunogen is located within amino acids 210-260 of PD-1.

Research Area: Apoptosis

Tested Application: E, WB, IHC-P, IF

Application: WB: 1-4 μg/mL; IHC: 2.5-5 μg/mL; IF: 20 μg/mL.
Antibody validated: Western Blot in human, mouse and rat samples; Immunohistochemistry in human samples; Immunofluorescence in human samples. All other applications and species not yet tested.

Specificiy: N/A

Positive Control 1: Cat. No. 1204 - K562 Cell Lysate

Positive Control 2: Cat. No. 1205 - Jurkat Cell Lysate

Positive Control 3: Cat. No. 1208 - THP-1 Cell Lysate

Positive Control 4: Cat. No. 10-301 - Human Brain Tissue Slide

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: Human PD-1 has 1 isoform (288aa, 32kD) . Mouse PD-1 has 1 isoform 288aa, 32kD) and Rat PD-1 also has one isoform (287aa, 32kD) . 4065 can detect human, mouse and rat.

Validation:

KO Validation (Figure 1) : Anti-PD-1 antibodies (4065) specificity was further verified by PD-1 specific knockout. PD-1 signal was not detected in PD-1 knockout HeLa cells as compared to that in control wild type cells.

KD validation (Figure 2) : Anti-PD-1 antibody (4065) specificity was further verified by PD-1 specific knockdown. PD-1 signal in HeLa cells transfected with PD-1 siRNAs or shRNA was disrupted in comparison with that in cells transfected with control siRNAs.

Isoform: Human PD-1 has 1 isoform (288aa, 32kD) . Mouse PD-1 has 1 isoform 288aa, 32kD) and Rat PD-1 also has one isoform (287aa, 32kD) . 4065 can detect human, mouse and rat.

Purification: PD-1 Antibody is affinity chromatography purified via peptide column.

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: PD-1 Antibody is supplied in PBS containing 0.02% sodium azide.

Concentration: 1 mg/mL

Storage Condition: PD-1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Alternate Name: PD-1 Antibody: PD1, PD-1, CD279, SLEB2, hPD-1, hPD-l, hSLE1, PD1, Programmed cell death protein 1, Protein PD-1, PDCD1, PDCD-1

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: PD-1 Antibody: Cell-mediated immune responses are initiated by T lymphocytes that are themselves stimulated by cognate peptides bound to MHC molecules on antig en-presenting cells (APC) . T-cell activation is generally self-limited as activated T cells express receptors such as PD-1 (also known as PDCD-1) that mediate inhibitory signals from the APC. PD-1 can bind two different but related ligands, PDL-1 and PDL-2. Upon binding to either of these ligands, signals generated by PD-1 inhibit the activation of the immune response in the absence of "danger signals" such as LPS or other molecules associated with bacteria or other pathogens. Evidence for this is seen in PD1-null mice who exhibit hyperactivated immune systems and autoimmune diseases.