Elabscience
QuicKey Pro Human IGF-1 (Insulin-like Growth Factor 1) ELISA Kit
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- SKU:
- E-OSEL-H0012
- Weight:
- 0 KGS
- Shipping:
- Calculated at Checkout
Description
QuicKey Pro Series
Get more sensitive and precise results with saving at least 1-2h comparing to traditional ELISA Kits. The new developed technology in house will help to accelerate your science research in a more efficient way.
Product Details
Properties
| Assay type | Sandwich-ELISA |
| Format | 96T/48T/24 |
| Assay time | 1.5h |
| Detection range | 3.12-100 ng/mL |
| Sensitivity | 1.01 ng/mL |
| Sample type &Sample volume | serum and plasma; 50μL |
| Specificity | This kit recognizes IGF-1 in samples. No significant cross-reactivity or interference between IGF-1 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are < 10%. |
| Application | This ELISA kit applies to the in vitro quantitative determination of IGF-1 concentrations in serum and plasma. |
Test Principle
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human IGF-1. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Human IGF-1 are added to the micro ELISA plate wells. Human IGF-1 in samples (or standards) combines with the coated antibody and HRP linked detection antibody specific to IGF-1. Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450±2 nm. The concentration of Human IGF-1 in the samples is then determined by comparing the OD of the samples to the standard curve.
Kit components & Storage
An unopened kit can be stored at 2-8℃ for 6 months. After test, the unused wells and reagents should be stored according to the table.
| Item | Specifications | Storage |
|---|---|---|
| Micro ELISA Plate(Dismountable) | 96T: 8 wells ×12 strips 48T: 8 wells ×6 strips 24T: 8 wells ×3 strips 96T*5: 5 plates, 96T |
2-8℃, 1 months |
| Reference Standard | 96T: 2 vials 48T/24T: 1 vial 96T*5: 10 vials |
2-8℃, use the reconstituted standard within 24h |
| Concentrated HRP Linked Detection Ab(100×) | 96T: 1 vial, 60 μL 48T/24T: 1 vial, 30 μL 96T*5: 5 vials, 60 μL |
2-8℃(Protect from light) |
| Reference Standard & Sample Diluent | 96T/48T/24T: 1 vial, 20 mL 96T*5: 5 vials, 20 mL |
2-8℃ |
| HRP Linked Ab Diluent | 96T/48T/24T: 1 vial, 14 mL 96T*5: 5 vials, 14 mL |
|
| Concentrated Wash Buffer(25×) | 96T/48T/24T: 1 vial, 30 mL 96T*5: 5 vials, 30 mL |
|
| Substrate Reagent | 96T/48T/24T: 1 vial, 10 mL 96T*5: 5 vials, 10 mL |
2-8℃(Protect from light) |
| Stop Solution | 96T/48T/24T: 1 vial, 10 mL 96T*5: 5 vials, 10 mL |
2-8℃ |
| Plate Sealer | 96T/48T/24T: 5 pieces 96T*5: 25 pieces |
|
| Product Description | 1 copy | |
| Certificate of Analysis | 1 copy |
Technical Data
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level IGF-1 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level IGF-1 were tested on 3 different plates, 20 replicates in each plate.
| Intra-assay Precision | Inter-assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (ng/mL) | 108.85 | 204.73 | 939.71 | 108.24 | 188.86 | 876.39 |
| Standard deviation | 6.29 | 9.87 | 28.76 | 6.30 | 11.05 | 41.80 |
| CV (%) | 5.78 | 4.82 | 3.06 | 5.82 | 5.85 | 4.77 |
Recovery
The recovery of IGF-1 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.
| Sample Type | Range (%) | Average Recovery (%) |
|---|---|---|
| Serum(n=8) | 92-103 | 97 |
| EDTA plasma(n=8) | 90-104 | 95 |
Linearity
Samples were spiked with high concentrations of Human IGF-1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.
Target Information
| Database Links | SwissProt: P05019 |
| Synonyms | insulin-like growth factor 1,IGF1 |
| Research Area | Cancer, Developmental biology, Signal transduction |
Additional Information
Reactivity: |
Human |
Detection Range: |
3.12~100 ng/mL |
Sensitivity: |
1.01 ng/mL |
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