SHROOM1 Antibody

SHROOM1 Antibody | 7113 | ProSci

Host: Rabbit

Reactivity: Human, Mouse, Rat

Homology: N/A

Immunogen: Rabbit polyclonal SHROOM1 antibody was raised against a 16 amino acid peptide near the carboxy terminus of human SHROOM1.
The immunogen is located within the last 50 amino acids of SHROOM1.

Research Area: Stem Cell

Tested Application: E, WB

Application: SHROOM1 antibody can be used for detection of SHROOM1 by Western blot at 1 - 2 μg/mL.
Antibody validated: Western Blot in mouse samples. All other applications and species not yet tested.

Specificiy: SHROOM1 antibody is human, mouse and rat reactive. At least three isoforms of SHROOM1 are known to exist; this antibody will detect only the two largest isoforms. SHROOM1 antibody is predicted to not cross-react with other SHROOM family members.

Positive Control 1: Cat. No. 1403 - Mouse Brain Tissue Lysate

Positive Control 2: N/A

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: 94 kDa

Validation: N/A

Isoform: N/A

Purification: SHROOM1 Antibody is affinity chromatography purified via peptide column.

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: SHROOM1 Antibody is supplied in PBS containing 0.02% sodium azide.

Concentration: 1 mg/mL

Storage Condition: SHROOM1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Alternate Name: SHROOM1 Antibody: APXL2, APXL2, KIAA1960, Protein Shroom1, Apical protein 2

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: SHROOM1 Antibody: SHROOM family members play diverse roles in the development of the nervous system and other tissues. SHROOM1 is a PDZ domain-containing actin-binding protein that is required for neural tube morphogenesis. It facilitates neurulation by regulating the morphology of neurepithelial cells via the apical positioning of an actomyosin network in the neurepithelium. SHROOM1 is also expressed in pulmonary arterial smooth muscle cells and its expression is significantly decreased in mouse and human models of pulmonary arterial hypertension, suggesting that there may be a link between SHROOM1 expression and pulmonary arterial hypertension.