CXXC5 Antibody | 6111 | ProSci
Reactivity: Human, Mouse, Rat
Homology: Predicted species reactivity based on immunogen sequence: Bovine: (100%)
Immunogen: CXXC5 antibody was raised against a 15 amino acid synthetic peptide near the amino terminus of human CXXC5.
The immunogen is located within amino acids 30 - 80 of CXXC5.
Research Area: Stem Cell
Tested Application: E, WB, IHC-P, IF
Application: CXXC5 antibody can be used for detection of CXXC5 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Specificiy: At least two isoforms of CXXC5 are known to exist. CXXC5 antibody is predicted to not cross-react with other CXXC protein family members.
Positive Control 1: Cat. No. 1303 - Human Brain Tissue Lysate
Positive Control 2: Cat. No. 10-301 - Human Brain Tissue Slide
Positive Control 3: N/A
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: Predicted: 25, 35 kDa
Observed: 25, 35 kDa
Purification: CXXC5 Antibody is affinity chromatography purified via peptide column.
Physical State: Liquid
Buffer: CXXC5 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration: 1 mg/mL
Storage Condition: CXXC5 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Alternate Name: CXXC5 Antibody: CF5, WID, RINF, HSPC195, CXXC-type zinc finger protein 5, Putative MAPK-activating protein PM08, CF5
User Note: Optimal dilutions for each application to be determined by the researcher.
BACKGROUND: CXXC5 Antibody: CXXC5 is zinc finger protein closely related to the DVL1-binding protein CXXC4 (also known as Idax) , and like CXXC4 is thought to modulate the Wnt signaling pathway during development. CXXC5 is upregulated in neural stem cells upon BMP4 stimulation, leading to decreased levels of the Wnt-signaling target AXIN2 and facilitated the response of the stem cells to Wnt3a. Recent reports suggest CXXC5 transcription is activated by the Wilms tumor 1 (WT1) transcription factor, and together are involved in the WNT/beta-catenin pathway and play a role in embryonic kidney development.
E, IF, IHC-P, WB
Human, Mouse, Rat