LIF Antibody

LIF Antibody | 6245 | ProSci

Host: Rabbit

Reactivity: Human, Mouse, Rat

Homology: Predicted species reactivity based on immunogen sequence: Bovine: (100%)

Immunogen: Anti-LIF antibody (6245) was raised against a peptide corresponding to 16 amino acids near the center of human LIF.
The immunogen is located within amino acids 50-100 of LIF.

Research Area: Stem Cell

Tested Application: E, WB, IF

Application: WB: 1-4 μg/mL; IF: 20 μg/mL.
Antibody validated: Western Blot in human, mouse and rat samples; Immunofluorescence in mouse samples. All other applications and species not yet tested.

Specificiy: At least two isoforms of LIF are known to exist.

Positive Control 1: Cat. No. 1287 - EL4 Cell Lysate

Positive Control 2: Cat. No. 17-201 - 3T3/BALB Cell Slide

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: Predicted: 22kD
Observed: 26kD (Post-modifications: N-linked Glycosylations)

Validation:

Recombinant Protein Test (Figure 3) : Anit-LIF antibodies (6245) detected human LIF recombinant protein at different concentrations.

Isoform: Human LIF has 2 isoforms, including isoform 1 (202aa, 22kD) and isoform 2 (88aa, 9.6kD) . Mouse LIF has 1 isoform (203aa, 22.3kD and Rat LIF also has 1 isoform (202aa, 22.1kD) . 6245 can detect human isoform1 as well as mouse and rat LIF.

Purification: LIF Antibody is affinity chromatography purified via peptide column.

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: LIF Antibody is supplied in PBS containing 0.02% sodium azide.

Concentration: 1 mg/mL

Storage Condition: LIF antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Alternate Name: LIF Antibody: CDF, DIA, HILDA, MLPLI, Leukemia inhibitory factor, Differentiation-stimulating factor, LIF

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: LIF Antibody: LIF is a pleiotropic cytokine with roles in several different systems. It is involved in the induction of hematopoietic differentiation in normal and myeloid leukemia cells, induction of neuronal cell differentiation, regulator of mesenchymal to epithelial conversion during kidney development, and may also have a role in immune tolerance at the maternal-fetal interface. LIF was initially recognized by its ability to induce terminal differentiation of myeloid leukemic cells. It is a member of the IL-6 cytokine superfamily and can be highly glycosylated. LIF signaling is transduced through the LIF-R/gp130 receptor complex, leading to the phosphorylation and activation of the JAK/STAT pathway. Recent evidence shows that LIF inhibits cardiomyogenesis in embryonic stem cells via STAT3 activation.