B7H3 Antibody [4H3]

B7H3 Antibody [4H3] | RF16093 | ProSci

Host: Mouse

Reactivity: Human

Homology: N/A

Immunogen: B7-H3 antibody was raised against the extracellular domain of human B7-H3

Research Area: Immunology

Tested Application: E, WB, IHC-P, ICC, IF, Flow

Application: B7-H3 antibody can be used for detection of B7-H3 by Western blot at 0.25 μg/mL. Antibody can also be used for immunohistochemistry starting at 2 μg/mL and Immunocytochemistry starting at 1 μg/mL. For immunofluorescence start at 10 μg/mL. Flow cytometry at 1 μg/ml.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples; Immunocytochemistry in human samples; Immunofluorescence in human samples and Flow Cytometry in human samples. All other applications and species not yet tested.

Specificiy: N/A

Positive Control 1: Cat. No. 1210 - HEK293 Cell Lysate

Positive Control 2: Cat. No. 17-010 - HEK293 Cell Slide

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: Predicted: 57 kDa
Observed: 100 kDa

Validation: N/A

Isoform: N/A

Purification: B7-H3 Antibody is supplied as protein A purified IgG1.

Clonality: Monoclonal

Clone: 4H3

Isotype: IgG1, k

Conjugate: Unconjugated

Physical State: Liquid

Buffer: B7-H3 Antibody is supplied in PBS containing 0.02% sodium azide and 50% glycerol.

Concentration: 1 mg/mL

Storage Condition: B7-H3 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Alternate Name: B7-H3 Antibody: CD276 molecule, B7H3, B7RP-2, 4Ig-B7-H3, CD276

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: CD276, also known as B7-H3, was initially identified as a member of the B7 family of proteins through its homology with previously identified B7 molecules (1) . CD276 mRNA is widely expressed, but its protein expression is usually rather low (2) . CD276 has been shown to play a role in both the costimulation as well as the coinhibition of T cell response (3) . In a similar fashion, CD276 plays a critical role in the control of antitumor immune responses in some cases, while in others appears to mediate antitumor immunity (4) . It thus joins other immune checkpoint proteins as a possible therapeutic target for at least a subset of cancers.