VISTA Antibody [4C4]

VISTA Antibody [4C4] | RF16071 | ProSci

Host: Mouse

Reactivity: Human

Homology: N/A

Immunogen: VISTA antibody was raised against the extracellular domain of human VISTA.

Research Area: Immunology

Tested Application: E, WB, IHC-P, ICC, IF, Flow

Application: VISTA antibody can be used for detection of VISTA by Western blot at 0.25 - 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2 μg/mL and immunocytochemistry starting at 1 μg/mL. For immunofluorescence start at 2 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples; Immunocytochemistry in human samples; Immunofluorescence in human samples and Flow Cytometry in mouse samples. All other applications and species not yet tested.

Specificiy: N/A

Positive Control 1: Cat. No. 1331 - Human Lymphoma Tissue Lysate

Positive Control 2: Cat. No. 1306 - Human Spleen Tissue

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: Predicted: 31 kDa
Observed: 47 kDa

Validation: N/A

Isoform: N/A

Purification: VISTA Antibody is supplied as protein A purified IgG1.

Clonality: Monoclonal

Clone: 4C4

Isotype: IgG1

Conjugate: Unconjugated

Physical State: Liquid

Buffer: VISTA Antibody is supplied in PBS containing 0.02% sodium azide and 50% glycerol.

Concentration: 1 mg/mL

Storage Condition: VISTA antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Alternate Name: VISTA Antibody: VISTA molecule, VSIR, B7-H5, B7H5, GI24, PP2135, SISP1, DD1alpha, VISTA, C10orf54, chromosome 10 open reading frame 54, PD-1H, V-set immunoregulatory receptor, V-Type Immunoglobulin Domain-Containing Suppressor Of T-Cell Activation, Chromosome 10 Open Reading Frame 54

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: VISTA Antibody: VISTA/B7-H5/platelet receptor Gi24 is a single-pass type I membrane protein located at the cell surface. It is an immunoregulatory receptor which can inhibit T-cell response and may promote differentiation of embryonic stem cells, by inhibiting the BMP4 signaling pathway. The protein can be cleaved by MMP14, and stimulate MMP14-mediated MMP2 activation.