ProSci

VISTA Antibody [4C4]

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SKU:
RF16071
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  • VISTA Antibody [4C4]
  • VISTA Antibody [4C4]
  • VISTA Antibody [4C4]
  • VISTA Antibody [4C4]
  • VISTA Antibody [4C4]
  • VISTA Antibody [4C4]
  • VISTA Antibody [4C4]
  • VISTA Antibody [4C4]
  • VISTA Antibody [4C4]
  • VISTA Antibody [4C4]
  • VISTA Antibody [4C4]
  • VISTA Antibody [4C4]
  • VISTA Antibody [4C4]
€185.92 - €477.25

Description

VISTA Antibody [4C4] | RF16071 | ProSci

 

Host: Mouse

 

Reactivity: Human

 

Homology: N/A

 

Immunogen: VISTA antibody was raised against the extracellular domain of human VISTA.

 

Research Area: Immunology

 

Tested Application: E, WB, IHC-P, ICC, IF, Flow

 

Application: VISTA antibody can be used for detection of VISTA by Western blot at 0.25 - 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2 μg/mL and immunocytochemistry starting at 1 μg/mL. For immunofluorescence start at 2 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples; Immunocytochemistry in human samples; Immunofluorescence in human samples and Flow Cytometry in mouse samples. All other applications and species not yet tested.

 

Specificiy: N/A

 

Positive Control 1: Cat. No. 1331 - Human Lymphoma Tissue Lysate

 

Positive Control 2: Cat. No. 1306 - Human Spleen Tissue

 

Positive Control 3: N/A

 

Positive Control 4: N/A

 

Positive Control 5: N/A

 

Positive Control 6: N/A

 

Molecular Weight: Predicted: 31 kDa
Observed: 47 kDa

 

Validation: N/A

 

Isoform: N/A

 

Purification: VISTA Antibody is supplied as protein A purified IgG1.

 

Clonality: Monoclonal

 

Clone: 4C4

 

Isotype: IgG1

 

Conjugate: Unconjugated

 

Physical State: Liquid

 

Buffer: VISTA Antibody is supplied in PBS containing 0.02% sodium azide and 50% glycerol.

 

Concentration: 1 mg/mL

 

Storage Condition: VISTA antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

 

Alternate Name: VISTA Antibody: VISTA molecule, VSIR, B7-H5, B7H5, GI24, PP2135, SISP1, DD1alpha, VISTA, C10orf54, chromosome 10 open reading frame 54, PD-1H, V-set immunoregulatory receptor, V-Type Immunoglobulin Domain-Containing Suppressor Of T-Cell Activation, Chromosome 10 Open Reading Frame 54

 

User Note: Optimal dilutions for each application to be determined by the researcher.

 

BACKGROUND: VISTA Antibody: VISTA/B7-H5/platelet receptor Gi24 is a single-pass type I membrane protein located at the cell surface. It is an immunoregulatory receptor which can inhibit T-cell response and may promote differentiation of embryonic stem cells, by inhibiting the BMP4 signaling pathway. The protein can be cleaved by MMP14, and stimulate MMP14-mediated MMP2 activation.

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Additional Information

Clonality:
Monoclonal
Tested Applications:
E, Flow, ICC, IF, IHC-P, WB
Host Species:
Mouse
Species Reactivity:
Human
Conjugate:
Unconjugated
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