VISTA Antibody

VISTA Antibody | 8695 | ProSci

Host: Rabbit

Reactivity: Human

Homology: Predicted species reactivity based on immunogen sequence: Mouse: (93%)

Immunogen: VISTA antibody was raised against a peptide corresponding to 15 amino acids near the carboxy terminus of human VISTA.
The immunogen is located within the last 60 amino acids of VISTA.

Research Area: Immunology

Tested Application: E, WB, IHC, ICC, IF

Application: VISTA antibody can be used for Immunohistochemistry at 2 μg/mL. For Immunoflorescence start at 20 μg/mL. For Immunocytochemistry start at 1 μg/mL.
Antibody validated: Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Specificiy: N/A

Positive Control 1: N/A

Positive Control 2: Cat. No. 11-533 - Human Tonsil Tissue Slide

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: N/A

Validation: N/A

Isoform: N/A

Purification: VISTA Antibody is affinity chromatography purified via peptide column.

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: VISTA Antibody is supplied in PBS containing 0.02% sodium azide.

Concentration: 1 mg/mL

Storage Condition: VISTA antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Alternate Name: VISTA Antibody: VISTA molecule, VSIR, B7-H5, B7H5, GI24, PP2135, SISP1, DD1alpha, VISTA, C10orf54, chromosome 10 open reading frame 54, PD-1H, V-set immunoregulatory receptor, V-Type Immunoglobulin Domain-Containing Suppressor Of T-Cell Activation, Chromosome 10 Open Reading Frame 54

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: The lymphocyte activation gene-3 (LAG3) is a member of the immunoglobulin superfamily and binds MHC class II with high affinity (1) , negatively regulating T-cell function and homeostasis (2) . It is expressed in B, T, and NK cells, monocytes, and dendritic cells (3) , and acts to regulate T cell expansion (4) . LAG3 is also an important immune checkpoint protein, with anti-LAG3 antibodies activating T effector cells and affecting regulatory T cell functions. Furthermore LAG3 appears to act in a synergistic fashion with PD-1/PD-L1, suggesting that a dual antibody approach may prove useful in cancer immunotherapy (5) .