GABARAP Antibody

GABARAP Antibody | 7359 | ProSci

Host: Rabbit

Reactivity: Human, Mouse, Rat

Homology: Predicted species reactivity based on immunogen sequence: Rabbit: (100%) , Bovine: (100%)

Immunogen: GABARAP antibody was raised against a 19 amino acid peptide near the amino terminus of human GABARAP.
The immunogen is located within the first 50 amino acids of GABARAP.

Research Area: Autophagy

Tested Application: E, WB, IHC-P, IF

Application: GABARAP antibody can be used for detection of GABARAP by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in mouse samples and Immunofluorescence in human and mouse samples. All other applications and species not yet tested.

Specificiy: Multiple isoforms of GABARAP exist due to alternative splicing events.

Positive Control 1: Cat. No. 1201 - HeLa Cell Lysate

Positive Control 2: Cat. No. 10-301 - Human Brain Tissue Slide

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: Predicted: 13kDa
Observed: 15 kDa

Validation: N/A

Isoform: N/A

Purification: GABARAP Antibody is affinity chromatography purified via peptide column.

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: GABARAP Antibody is supplied in PBS containing 0.02% sodium azide.

Concentration: 1 mg/mL

Storage Condition: GABARAP antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.

Alternate Name: GABARAP Antibody: MM46, ATG8A, GABARAP-a, FLC3B, HT004, Gamma-aminobutyric acid receptor-associated protein, MM46

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: GABARAP Antibody: Gamma-aminobutyric acid (GABA) is the main inhibitory transmitter by increasing a Cl-conductance that inhibits neuronal firing in the central nervous system. It has been shown to activate both ionotropic (GABAA) and metabotropic (GABAB) receptors as well as a third class of receptors called GABAC. GABARAP (GABAA receptor-associated protein) links GABAA receptors to the cytoskeleton and may play a role in intracellular transport of GABAA receptors and its interaction with the cytoskeleton. GABARAP belongs to the MAP1 or ATG8 like family and recent studies show that MAPK15/ERK8 is acting through interaction with ATG8 family proteins to regulate autophagy.