GABARAPL2 Antibody

GABARAPL2 Antibody | 7895 | ProSci

Host: Rabbit

Reactivity: Human, Mouse, Rat

Homology: Predicted species reactivity based on immunogen sequence: Bovine: (100%)

Immunogen: GABARAPL2 antibody was raised against a 15 amino acid peptide near the carboxy terminus of human GABARAPL2.
The immunogen is located within the last 50 amino acids of GABARAPL2.

Research Area: Autophagy

Tested Application: E, WB, IHC-P, IF

Application: GABARAPL2 antibody can be used for detection of GABARAPL2 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry at 5 μg/mL. For Immunoflorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in mouse and rat samples and Immunofluorescence in mouse and rat samples. All other applications and species not yet tested.

Specificiy: GABARAPL2 antibody is human, mouse and rat reactive. Multiple isoforms of GABARAPL2 are known to exist.

Positive Control 1: Cat. No. 1303 - Human Brain Tissue Lysate

Positive Control 2: N/A

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: Predicted: 13 kDa
Observed: 13 kDa

Validation: N/A

Isoform: N/A

Purification: GABARAPL2 antibody is affinity chromatography purified via peptide column.

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: GABARAPL2 antibody is supplied in PBS containing 0.02% sodium azide.

Concentration: 1 mg/mL

Storage Condition: GABARAPL2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.

Alternate Name: GABARAPL2 Antibody: ATG8, GEF2, ATG8C, GEF-2, GATE16, GATE-16, FLC3A, Gamma-aminobutyric acid receptor-associated protein-like 2, Ganglioside expression factor 2

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: Gamma-aminobutyric acid (GABA) is the main inhibitory transmitter by increasing a Cl-conductance that inhibits neuronal firing in the central nervous system (1) . It has been shown to activate both ionotropic (GABAA) and metabotropic (GABAB) receptors as well as a third class of receptors called GABAC (2) . GABARAPL2 (GABAA receptor-associated protein-like 2) , also known as GATE16, was initially identified as a membrane transport modulator and is a mammalian ortholog to the autophagy protein ATG8 (3, 4) . It is thought that GABARAPL2 and other members of the ATG8 family act as scaffolds for assembly of the Unc-51 like kinase (ULK) complex in the formation of autophagosomes (5) .