FY-EH4497 Human IGFBP-3(Insulin-like growth factor-binding protein 3) ELISA Kit

IGFBP-3(Insulin-like growth factor-binding protein 3) Basic information

IGFBP-3 was first isolated, characterized, and quantitated in human plasma, in 1986. It has well-documented functions in the circulation, in the extracellular environment, and inside cells. It is the main IGF transport protein in the bloodstream, where it carries the growth factors predominantly in stable complexes that contain the binding protein, either IGF-1 or IGF-2, and a third protein called the acid-labile subunit or ALS. For IGFs to reach the tissues from the bloodstream, the circulating complexes are believed to partly dissociate, possibly enhanced by limited proteolysis of IGFBP-3. The IGF-1/IGFBP-3 ratio has sometimes been used as an index of IGF bioavailability in the human circulation, but this ignores IGF-1 binding to other IGFBPs (so the ratio is affected by the concentrations of all six IGFBPs), and the fact that IGF-2, which is three times more abundant than IGF-1 in the bloodstream of adults, occupies the majority of binding sites on circulating IGFBP-3.

Human IGFBP-3(Insulin-like growth factor-binding protein 3) ELISA Kit test method

Feiyue’s Human IGFBP-3(Insulin-like growth factor-binding protein 3) is an ELISA reagent for detection of IGFBP-3(Insulin-like growth factor-binding protein 3) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

This kit uses sandwich ELISA to detect the concentration of Human IGFBP-3(Insulin-like growth factor-binding protein 3). Human IGFBP-3(Insulin-like growth factor-binding protein 3)-specific monoclonal antibody has been pre-coated in the wells of the supplied microplate. Standards samples and controls are added to interact with the immobilized antibody. A sandwich complex is formed by additional anti-Human IGFBP-3(Insulin-like growth factor-binding protein 3) antibody with HRP-Streptavidin. TMB solution is added to react with the sandwich for ming optical signal measured by microplate reader. The concentration of Human IGFBP-3(Insulin-like growth factor-binding protein 3) in the sample can be calculated by comparing the absorbance of the sample with the standard curve.

Reference:

1. GRCh38: Ensembl release 89: ENSG00000146674 - Ensembl, May 2017

2. GRCm38: Ensembl release 89: ENSMUSG00000020427 - Ensembl, May 2017

3. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.

4. "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.