Pembrolizumab ELISA (KEYTRUDA)

The Pembrolizumab (Keytruda) ELISA kit is intended for the quantitative determination of Pembrolizumab (Keytruda) in serum, plasma and cell culture samples. RUO only.

Intended Use:
The KRIBIOLISA™ Pembrolizumab (Keytruda™) ELISA is used for quantitative detection of Pembrolizumab in serum and plasma.
 

Principle:
The method employs the quantitative enzyme immunoassay technique. Standards or samples are pipetted into microwells pre-coated with Anti- Pembrolizumab antibody and Pembrolizumab present in the sample and standards are bound by Anti- Pembrolizumab antibody. In the second step, Detection antidody is added and incubated. In the third step, a HRP conjugate is pipetted and incubated. Free HRP conjugate will be removed by washing. Addition of TMB substrate will develop blue color and intensity of blue colour in wells is proportional to the concentration of Pembrolizumab present in standard or sample. Color development is then stopped by addition of stop solution. Absorbance is measured at 450 nm.