Elabscience
Hydrogen Peroxide (H2O2) Colorimetric Assay Kit
- SKU:
- E-BC-K102-M
- Weight:
- 0 KGS
- Shipping:
- Calculated at Checkout
Description
Detection principle
Hydrogen peroxide can react with ammonium molybdate to form a yellow complex which has a maximum absorption peak at 405 nm. H2O2 content can be calculated by measuring the absorbance value at 405 nm.
Performance characteristics
| Synonyms | H2O2 |
| Sample type | Serum,plasma,urine,tissue,cells |
| Sensitivity | 0.41 mmol/L |
| Detection range | 0.41-125 mmol/L |
| Detection method | Colorimetric method |
| Assay type | Quantitative |
| Assay time | 30 min |
| Precision | Average inter-assay CV: 3.6%Average intra-assay CV: 3.2% |
| Other instruments required | Micropipettor, Centrifuge, Incubator, Vortex mixer |
| Other reagents required | Normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4) |
| Storage | 2-8℃ |
| Valid period | 6 months |
Images
Electrical stimulation for more than 8 hours could lead to a significant increase of H2O2 in the media.
S Du et al investigate the promotion effect of wounds healing using programmed electric field together with minocycline. Hydrogen peroxide (H2O2) level in bacteria NB media was determined using H2O2 colorimetric assay kit (E-BC-K102-M).
Dilution of sample
It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.41-125 mmol/L).
The recommended dilution factor for different samples is as follows (for reference only):
|
Sample type |
Dilution factor |
|
Human serum |
1 |
|
Rat serum |
1 |
|
Mouse serum |
1 |
|
Mouse plasma |
1 |
|
Porcine serum |
1 |
|
Human urine |
1 |
|
Cell homogenate |
1 |
|
10% Plant tissue homogenate |
1 |
|
10% Rat heart tissue homogenate |
1 |
|
10% Rat brain tissue homogenate |
1 |
|
10% Rat kidney tissue homogenate |
1 |
|
10% Rat liver tissue homogenate |
1 |
Note: The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4).
Additional Information
Detection method: |
Colorimetric method |
Detection instrument: |
Microplate reader(402-407 nm,optimum wavelength: 405 n |
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