Iron Colorimetric Assay Kit

Detection principle

Under the action of acidic solution and reductant, ferric ions can be separated from transferrin in serum, and reduced into ferrous ions (Fe2+). The latter then bind to bipyridine and form pink complexes. The concentration of iron can be calculated by measuring the OD value at 520 nm indirectly.

Performance characteristics

Images

The serum iron levels were decreased in all MC-LR treatment groups (A), mice with MC-LR exposure developed iron overload in different tissue (B-F).

C Pan et al investigate risk of microcytic anemia through chronic exposure to MC-LR. Iron (Fe) level in mouse serum and tissue were determined using iron colorimetric assay kit (E-BC-K139-S).

Dilution of sample

It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.072-60 mg/L).

The recommended dilution factor for different samples is as follows (for reference only):

Note: The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4).