Elabscience

Nitric Oxide (NO) Colorimetric Assay Kit

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E-BC-K035-M
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  • Nitric Oxide (NO) Colorimetric Assay Kit
  • Nitric Oxide (NO) Colorimetric Assay Kit
  • Nitric Oxide (NO) Colorimetric Assay Kit
€256.00 - €295.00

Description

Detection principle

NO is easily oxidized to form NO2- in vivo or in aqueous solution, and a reddish azo compound is formed with the color developing agent, and the concentration of the azo compound is linearly related to the concentration of NO. The concentration of NO can be calculated indirectly by measuring the OD value at 550 nm.

Performance characteristics

Synonyms NO
Sample type Serum, plasma, tissue, saliva
Sensitivity 0.16 μmol/L
Detection range 0.16-100 μmol/L
Detection method Colorimetric method
Assay type Quantitative
Assay time 55 min
Precision Average inter-assay CV: 3.7%Average intra-assay CV: 2.4%
Other instruments required Micropipettor, Vortex mixer, Centrifuge
Other reagents required Normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4)
Storage Reagent 6: -20℃, others: 2-8℃
Valid period 6 months

Dilution of sample

It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.16-100 μmol/L).

The recommended dilution factor for different samples is as follows (for reference only):

Sample type

Dilution factor

Human serum

1

Human plasma

1

10% Mouse liver tissue homogenization

1

Rat serum

1

Rat plasma

1

10% Epipremnum aureum tissue homogenization

1

Note: The diluent is double distilled water, normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4).

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Additional Information

Detection method:
Colorimetric method
Detection instrument:
Microplate reader(540-550 nm)
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