Elabscience
Lipid Peroxide (LPO) Colorimetric Assay Kit
- SKU:
- E-BC-K176-M
- Weight:
- 0 KGS
- Shipping:
- Calculated at Checkout
Description
Detection principle
With 45℃ incubation for 60 min, one molecule of LPO react with two molecule of chromogenic reagent, to produce a stable chromophore which have the maximum absorption peak at 586nm. The content of LPO in samples can be calculated by standard curve or calculation formula.
Performance characteristics
| Synonyms | LPO |
| Sample type | Serum,plasma,urine,tissue |
| Sensitivity | 0.70 μmol/L |
| Detection range | 0.70-80 μmol/L |
| Detection method | Colorimetric method |
| Assay type | Quantitative |
| Assay time | 90 min |
| Precision | Average inter-assay CV: 3.5%Average intra-assay CV: 3.1% |
| Other instruments required | Water bath, Centrifuge, Micropipettor, Vortex mixer |
| Other reagents required | Double distilled water, PBS (0.01 M, pH 7.4) |
| Storage | 2-8℃ |
| Valid period | 6 months |
Dilution of sample
It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range ( 0.70-80 μmol/L).
The recommended dilution factor for different samples is as follows (for reference only):
|
Sample type |
Dilution factor |
|
Human serum |
1 |
|
Human plasma |
1 |
|
Urine |
1 |
|
10% Rat kidney tissue homogenization |
1 |
|
10% Rat liver tissue homogenization |
1 |
Note: The diluent is PBS (0.01 M, pH 7.4).
Additional Information
Detection instrument: |
Microplate reader(580-590 nm) |
Detection method: |
Colorimetric method |
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