Elabscience

Lipid Peroxide (LPO) Colorimetric Assay Kit

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E-BC-K176-M
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  • Lipid Peroxide (LPO) Colorimetric Assay Kit
  • Lipid Peroxide (LPO) Colorimetric Assay Kit
  • Lipid Peroxide (LPO) Colorimetric Assay Kit
€438.00 - €594.00

Description

Detection principle

With 45℃ incubation for 60 min, one molecule of LPO react with two molecule of chromogenic reagent, to produce a stable chromophore which have the maximum absorption peak at 586nm. The content of LPO in samples can be calculated by standard curve or calculation formula.

Performance characteristics

Synonyms LPO
Sample type Serum,plasma,urine,tissue
Sensitivity 0.70 μmol/L
Detection range 0.70-80 μmol/L
Detection method Colorimetric method
Assay type Quantitative
Assay time 90 min
Precision Average inter-assay CV: 3.5%Average intra-assay CV: 3.1%
Other instruments required Water bath, Centrifuge, Micropipettor, Vortex mixer
Other reagents required Double distilled water, PBS (0.01 M, pH 7.4)
Storage 2-8℃
Valid period 6 months

Dilution of sample

It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range ( 0.70-80 μmol/L).

The recommended dilution factor for different samples is as follows (for reference only):

Sample type

Dilution factor

Human serum

1

Human plasma

1

Urine

1

10% Rat kidney tissue homogenization

1

10% Rat liver tissue homogenization

1

Note: The diluent is PBS (0.01 M, pH 7.4).

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Additional Information

Detection instrument:
Microplate reader(580-590 nm)
Detection method:
Colorimetric method
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