Elabscience
Myeloperoxidase (MPO) Activity Assay Kit
- SKU:
- E-BC-K074-M
- Weight:
- 0 KGS
- Shipping:
- Calculated at Checkout
Description
Detection principle
Myeloperoxidase reduces hydrogen peroxide to a complex. The complex react with o-dianisidine (as hydrogen donor) to produce a yellow product which has a maximum absorption peak at 460 nm. The activity of MPO can be calculated indirectly by measuring the OD value at 460nm.
Performance characteristics
| Synonyms | MPO |
| Sample type | Serum, plasma, animal tissue |
| Sensitivity | 19.42 U/L |
| Detection range | 19.42-893.31 U/L |
| Detection method | Colorimetric method |
| Assay type | Enzyme Activity |
| Assay time | 65 min |
| Precision | Average inter-assay CV: 7.3%Average intra-assay CV: 5.4% |
| Other instruments required | Tubes, Micropipette, Vortex mixer, 37℃/60℃ Water bath |
| Storage | 2-8℃ |
| Valid period | 6 months |
Images
The activity of MPO indicating the neutrophil level was slightly higher in the KO (C3 knockout) than WT (wild type) mice.
Y J Choi investigate the effective of complement component 3 (C3) deficiency promoting its receptor downstream-mediated inflammatory response. Myeloperoxidase (MPO) activity of mouse colon was determined using MPO activity assay kit (E-BC-K074-M).
Dilution of sample
It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (19.42-893.31 U/L).
The recommended dilution factor for different samples is as follows (for reference only):
|
Sample type |
Dilution factor |
|
Human serum |
1 |
|
Human plasma |
1 |
|
Human milk |
1 |
|
Cell culture supernatant |
1 |
|
Rat serum |
1 |
|
Rat plasma |
1 |
|
5% Rat kidney tissue homogenate |
1 |
|
5% Rat spleen tissue homogenate |
1 |
Note: The diluent is reagent 2 application solution.
Additional Information
Detection method: |
Colorimetric method |
Detection instrument: |
Microplate reader(450 nm-470 nm,optimum wavelength: 460 nm) |
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