Myeloperoxidase (MPO) Activity Assay Kit

Detection principle

Myeloperoxidase reduces hydrogen peroxide to a complex. The complex react with o-dianisidine (as hydrogen donor) to produce a yellow product which has a maximum absorption peak at 460 nm. The activity of MPO can be calculated indirectly by measuring the OD value at 460nm.

Performance characteristics

Images

The activity of MPO indicating the neutrophil level was slightly higher in the KO (C3 knockout) than WT (wild type) mice.

Y J Choi investigate the effective of complement component 3 (C3) deficiency promoting its receptor downstream-mediated inflammatory response. Myeloperoxidase (MPO) activity of mouse colon was determined using MPO activity assay kit (E-BC-K074-M).

Dilution of sample

It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (19.42-893.31 U/L).

The recommended dilution factor for different samples is as follows (for reference only):

Note: The diluent is reagent 2 application solution.