Elabscience
Catalase (CAT) Activity Assay Kit
- SKU:
- E-BC-K031-M
- Weight:
- 0 KGS
- Shipping:
- Calculated at Checkout
Description
Detection principle
The reaction that catalase (CAT) decomposes H2O2 can be quickly stopped by ammonium molybdate. The residual H2O2 reacts with ammonium molybdate to generate a yellowish complex. CAT activity can be calculated by production of the yellowish complex at 405 nm.
Performance characteristics
| Synonyms | CAT |
| Sample type | Serum,plasma,cells,cell culture supernatant,tissue |
| Sensitivity | 1.12 U/mL |
| Detection range | 1.12 -150 U/mL |
| Detection method | Colorimetric method |
| Assay type | Enzyme Activity |
| Assay time | 40 min |
| Precision | Average inter-assay CV: 7.7%Average intra-assay CV: 3.9% |
| Other instruments required | Micropipettor, Vortex Mixer, Incubator |
| Other reagents required | Normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4) |
| Storage | 2-8℃ |
| Valid period | 6 months |
Images
The activity of CAT increased at the beginning of the UV exposure until 80 mJ/cm2, then decreased with the UV fluence reached 120 mJ/cm2. The induced CAT activity was higher in the UV/Cl2 process (purple) compared with that of the UV alone process.
Q Wan et al investigate the efficiency and mechanism of fungal spore disinfection using UV-LEDs irradiation and chlorine. Catalase (CAT) activity of fungal spore was determined using CAT activity assay kit (E-BC-K031-M).
Dilution of sample
It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (1.12 -150 U/mL).
The recommended dilution factor for different samples is as follows (for reference only):
|
Sample type |
Dilution factor |
|
Human serum |
1 |
|
293T supernatant |
1 |
|
10% Rat heart tissue homogenization |
50-100 |
|
10% Rat liver tissue homogenization |
100-200 |
|
10% Rat spleen tissue homogenization |
50-100 |
|
Mouse serum |
1 |
|
10% Epipremnum aureum tissue homogenization |
1-2 |
|
10% Rat lung tissue homogenization |
50-100 |
|
10% Rat kidney tissue homogenization |
50-100 |
|
10% Rat brain tissue homogenization |
20-50 |
Note: The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4).
Additional Information
Detection method: |
Colorimetric method |
Detection instrument: |
Microplate reader(400-410 nm,optimum wavelength: 405 nm) |
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ENLIBIO
