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Elabscience

Catalase (CAT) Activity Assay Kit

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SKU:
E-BC-K031-M
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  • Catalase (CAT) Activity Assay Kit
  • Catalase (CAT) Activity Assay Kit
  • Catalase (CAT) Activity Assay Kit
  • Catalase (CAT) Activity Assay Kit
  • Catalase (CAT) Activity Assay Kit
€256.00 - €295.00

Description

Detection principle

The reaction that catalase (CAT) decomposes H2O2 can be quickly stopped by ammonium molybdate. The residual H2O2 reacts with ammonium molybdate to generate a yellowish complex. CAT activity can be calculated by production of the yellowish complex at 405 nm.

Performance characteristics

Synonyms CAT
Sample type Serum,plasma,cells,cell culture supernatant,tissue
Sensitivity 1.12 U/mL
Detection range 1.12 -150 U/mL
Detection method Colorimetric method
Assay type Enzyme Activity
Assay time 40 min
Precision Average inter-assay CV: 7.7%Average intra-assay CV: 3.9%
Other instruments required Micropipettor, Vortex Mixer, Incubator
Other reagents required Normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4)
Storage 2-8℃
Valid period 6 months

Images

The activity of CAT increased at the beginning of the UV exposure until 80 mJ/cm2, then decreased with the UV fluence reached 120 mJ/cm2. The induced CAT activity was higher in the UV/Cl2 process (purple) compared with that of the UV alone process.

Q Wan et al investigate the efficiency and mechanism of fungal spore disinfection using UV-LEDs irradiation and chlorine. Catalase (CAT) activity of fungal spore was determined using CAT activity assay kit (E-BC-K031-M).

Dilution of sample

It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (1.12 -150 U/mL).

The recommended dilution factor for different samples is as follows (for reference only):

Sample type

Dilution factor

Human serum

1

293T supernatant

1

10% Rat heart tissue homogenization

50-100

10% Rat liver tissue homogenization

100-200

10% Rat spleen tissue homogenization

50-100

Mouse serum

1

10% Epipremnum aureum tissue homogenization

1-2

10% Rat lung tissue homogenization

50-100

10% Rat kidney tissue homogenization

50-100

10% Rat brain tissue homogenization

20-50

Note: The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4).

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Additional Information

Detection method:
Colorimetric method
Detection instrument:
Microplate reader(400-410 nm,optimum wavelength: 405 nm)
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