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Elabscience

Catalase (CAT) Activity Fluorometric Assay Kit

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E-BC-F006
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  • Catalase (CAT) Activity Fluorometric Assay Kit
  • Catalase (CAT) Activity Fluorometric Assay Kit
  • Catalase (CAT) Activity Fluorometric Assay Kit
€490.00

Description

Detection principle

Catalase can decompose H2O2 to generate H2O and O2, the residual H2O2 in the detection system react with the fluorescent substance, and the content of residual H2O2 is proportional to the fluorescence intensity at the excitation wavelength of 535 nm and emission wavelength of 587 nm, the catalase activity is inversely proportional to the fluorescence intensity.

Performance characteristics

Synonyms CAT
Sample type Serum, plasma, animal tissue
Sensitivity 0.01 U/L
Detection range 0.01-6.51 U/L
Detection method Fluorescence method
Assay type Enzyme Activity
Assay time 30 min
Precision Average inter-assay CV: 6.8%Average intra-assay CV: 3.4%
Other instruments required Vortex mixer, Centrifuge
Storage -20℃
Valid period 6 months

Dilution of sample

It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.01-6.51 U/L).

The recommended dilution factor for different samples is as follows (for reference only):

Sample type

Dilution factor

Mouse serum

60-80

Mouse plasma

60-80

Rat serum

70-80

Human saliva

30-50

Rat urine

30-50

10% Mouse liver tissue homogenate

2500-3000

10% Mouse lung tissue homogenate

200-400

10% Rat muscle tissue homogenate

100-200

10% Rat brain tissue homogenate

40-50

10% Mouse kidney tissue homogenate

2000-2500

Note: The diluent is reagent 1.

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Additional Information

Detection method:
Fluorescence method
Detection instrument:
Fluorescence microplate reader (Ex/Em=535 nm/587 nm)
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