Elabscience
Ascorbate Peroxidase (APX) Activity Assay Kit
- SKU:
- E-BC-K353-S
- Weight:
- 0 KGS
- Shipping:
- Calculated at Checkout
Description
Detection principle
Ascorbate Peroxidase (APX) can catalyze the reaction between ascorbic acid (ASA) and hydrogen peroxide (H2O2), and ASA can be oxidized to monodehydroascorbic acid (MDASA). The absorbance of solution at 290 nm will decline as the oxidation of ASA. The APX activity can be calculated by detecting the decrease of A290.
Performance characteristics
| Synonyms | APX |
| Sample type | Plant tissue |
| Sensitivity | 0.071 U/g tissue |
| Detection range | 0.071-47 U/g tissue |
| Detection method | Colorimetric method |
| Assay type | Enzyme Activity |
| Assay time | 60 min |
| Precision | Average inter-assay CV: 6.4%Average intra-assay CV: 4.8% |
| Other instruments required | Vortex mixer, Micropipettor, Water bath, Incubator, Centrifuge |
| Other reagents required | Normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4) |
| Storage | 2-8℃ |
| Valid period | 6 months |
Dilution of sample
It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.071-47 U/g tissue).
The recommended dilution factor for different samples is as follows (for reference only):
|
Sample type |
Dilution factor |
|
10% Epipremnum aureum tissue homogenization |
1 |
|
10% Carrot tissue homogenization |
1 |
|
10% Green pepper tissue homogenization |
1 |
|
10% Mushrooms tissue homogenization |
1 |
Note: The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4).
Additional Information
Detection instrument: |
Spectrophotometer(290 nm) |
Detection method: |
Colorimetric method |
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