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Elabscience

Peroxidase (POD) Activity Assay Kit (Plant samples)

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SKU:
E-BC-K227-M
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  • Peroxidase (POD) Activity Assay Kit (Plant samples)
  • Peroxidase (POD) Activity Assay Kit (Plant samples)
  • Peroxidase (POD) Activity Assay Kit (Plant samples)
€308.00 - €412.00

Description

Detection principle

Plant peroxidase, a member of the superfamily of peroxidase, catalyzes the redox reaction between H2O2 and various reductants. The plant peroxidase has the same general structure and consists of iron porphyrin IX and ten α-helixes. Based on the difference of primary structure, the superfamily of plant peroxidase can be divided into three types: class I (intracellular type), class II (extracellular type of fungi) and class III (secreted type of plant).

Performance characteristics

Synonyms POD
Sample type Plant tissue
Sensitivity 0.01 U/mL
Detection range 0.01–100 U/mL
Detection method Colorimetric method
Assay type Enzyme Activity
Assay time 60 min
Precision Average inter-assay CV: 5%Average intra-assay CV: 3.2%
Other instruments required Spectrophotometer (240 nm), Micropipettor, Vortex mixer, Centrifuge, 37℃ Incubator
Other reagents required PBS (0.01 M, pH 7.4)
Storage 2-8℃
Valid period 6 months

Dilution of sample

It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.01-100 U/mL).

The recommended dilution factor for different samples is as follows (for reference only):

Sample type

Dilution factor

10% Green pepper tissue homogenate

1

10% Chive leaf tissue homogenate

1

10% Photinia leaf tissue homogenate

1

10% Epipremnum aureum tissue homogenate

1

10% Mushrooms tissue homogenate

1

10% White radish tissue homogenate

1

Note: The diluent is PBS (0.01 M, pH 7.4).

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Additional Information

Detection method:
Colorimetric method
Detection instrument:
Microplate reader(410 nm-430 nm,optimum wavelength: 420 nm)
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