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Elabscience

Total Phenols Colorimetric Assay Kit (Plant samples)

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E-BC-K354-M
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  • Total Phenols Colorimetric Assay Kit (Plant samples)
  • Total Phenols Colorimetric Assay Kit (Plant samples)
  • Total Phenols Colorimetric Assay Kit (Plant samples)
€412.00

Description

Detection principle

Plant total phenol is a common secondary natural metabolite in plants. There are several kinds of phenolic compounds, such as hydroxybenzoic acid, hydroxy cinnamic acid, flavonoids, chalcone, flavonoids, lignin, coumarin and astragalus. Phenolic compounds are antioxidants that delay or prevent oxidation and oxygen radical reactions.

Performance characteristics

Sample type Plant tissue
Sensitivity 1.05 μg/mL
Detection range 1.05–148 μg/mL
Detection method Colorimetric method
Assay type Quantitative
Assay time 35 min
Precision Average inter-assay CV: 4.4%Average intra-assay CV: 4.1%
Other instruments required Incubator, Vacuum drying oven, Centrifuge
Other reagents required 60% alcohol
Storage 2-8℃
Valid period 6 months

Dilution of sample

It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (1.05-148 μg/mL).

The recommended dilution factor for different samples is as follows (for reference only):

Sample type

Dilution factor

Epipremnum aureum tissue homogenate

20-30

Daucus carota tissue homogenate

5-15

Spinacia oleracea tissue homogenate

15-25

Leek tissue homogenate

10-20

Note: The diluent is 60% alcohol.

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Additional Information

Detection instrument:
Microplate reader(750 nm-770 nm,optimum wavelength: 760 nm)
Detection method:
Colorimetric method
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