Elabscience
Total Phenols Colorimetric Assay Kit (Plant samples)
- SKU:
- E-BC-K354-M
- Weight:
- 0 KGS
- Shipping:
- Calculated at Checkout
Description
Detection principle
Plant total phenol is a common secondary natural metabolite in plants. There are several kinds of phenolic compounds, such as hydroxybenzoic acid, hydroxy cinnamic acid, flavonoids, chalcone, flavonoids, lignin, coumarin and astragalus. Phenolic compounds are antioxidants that delay or prevent oxidation and oxygen radical reactions.
Performance characteristics
| Sample type | Plant tissue |
| Sensitivity | 1.05 μg/mL |
| Detection range | 1.05–148 μg/mL |
| Detection method | Colorimetric method |
| Assay type | Quantitative |
| Assay time | 35 min |
| Precision | Average inter-assay CV: 4.4%Average intra-assay CV: 4.1% |
| Other instruments required | Incubator, Vacuum drying oven, Centrifuge |
| Other reagents required | 60% alcohol |
| Storage | 2-8℃ |
| Valid period | 6 months |
Dilution of sample
It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (1.05-148 μg/mL).
The recommended dilution factor for different samples is as follows (for reference only):
|
Sample type |
Dilution factor |
|
Epipremnum aureum tissue homogenate |
20-30 |
|
Daucus carota tissue homogenate |
5-15 |
|
Spinacia oleracea tissue homogenate |
15-25 |
|
Leek tissue homogenate |
10-20 |
Note: The diluent is 60% alcohol.
Additional Information
Detection instrument: |
Microplate reader(750 nm-770 nm,optimum wavelength: 760 nm) |
Detection method: |
Colorimetric method |
Related Products
Malondialdehyde (MDA) Colorimetric Assay Kit (Plant Samples)
Elabscience
Plant Flavonoids Colorimetric Assay Kit
Elabscience
Total Oxidant Status (TOS) Colorimetric Assay Kit
Elabscience
Total Antioxidant Status (TAS) Colorimetric Assay Kit
Elabscience
Iron Colorimetric Assay Kit
Elabscience