Elabscience
Total Antioxidant Status (TAS) Colorimetric Assay Kit
- SKU:
- E-BC-K801-M
- Weight:
- 0 KGS
- Shipping:
- Calculated at Checkout
Description
Detection principle
ABTS is oxidized to green ABTS•+ by appropriate oxidant, which can be reduced to colorless ABTS in the presence of antioxidants. The TAS of the sample can be determined and calculated by measuring the absorbance of ABTS•+ at 660 nm. Trolox is an analog of VE and has a similar antioxidant state to that of VE. Trolox is used as a reference substance for total antioxidant status.
Performance characteristics
| Synonyms | TAS |
| Sample type | serum, plasma, urine, cellular supernatant, animal, plant tissue |
| Sensitivity | 0.23 mmol Trolox Equiv./L |
| Detection range | 0.23-2 mmol Trolox Equiv./L |
| Detection method | Colorimetric method |
| Assay type | Quantitative |
| Assay time | 30 min |
| Precision | Average inter-assay CV: 7%Average intra-assay CV: 4.6% |
| Other instruments required | Micropipettor, 37℃ incubator. |
| Other reagents required | 60% Ethanol |
| Storage | -20℃ |
| Valid period | 6 months |
Images
TAS significantly decreased in diabetic model (DM), treatment of Cellgevity® significantly improved TAS. (**P<0.01, ***P<0.001)
O O Ogunlabi et al investigate the effects of Cellgevity® in diabetic model. Total antioxidants status (TAS) of rat serum was determined using TAS colorimetric assay kit (E-BC-K801-M).
Dilution of sample
It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.23-2 mmol Trolox Equiv. /L).
The recommended dilution factor for different samples is as follows (for reference only):
|
Sample type |
Dilution factor |
|
10% Mouse liver tissue homogenate |
1 |
|
10% Rat liver tissue homogenate |
1 |
|
10% Rat lung tissue homogenate |
1 |
|
Molt4 cellar supernatant |
1 |
|
Human urine |
8-10 |
|
Mouse serum |
1 |
|
Human serum |
1 |
|
Human saliva |
1 |
Note: The diluent is 60% Ethanol.
Additional Information
Detection instrument: |
Microplate reader(650 nm-670 nm,optimum wavelength: 660 nm) |
Detection method: |
Colorimetric method |
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