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Elabscience

Total Antioxidant Status (TAS) Colorimetric Assay Kit

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E-BC-K801-M
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  • Total Antioxidant Status (TAS) Colorimetric Assay Kit
  • Total Antioxidant Status (TAS) Colorimetric Assay Kit
  • Total Antioxidant Status (TAS) Colorimetric Assay Kit
€230.00 - €295.00

Description

Detection principle

ABTS is oxidized to green ABTS•+ by appropriate oxidant, which can be reduced to colorless ABTS in the presence of antioxidants. The TAS of the sample can be determined and calculated by measuring the absorbance of ABTS•+ at 660 nm. Trolox is an analog of VE and has a similar antioxidant state to that of VE. Trolox is used as a reference substance for total antioxidant status.

Performance characteristics

Synonyms TAS
Sample type serum, plasma, urine, cellular supernatant, animal, plant tissue
Sensitivity 0.23 mmol Trolox Equiv./L
Detection range 0.23-2 mmol Trolox Equiv./L
Detection method Colorimetric method
Assay type Quantitative
Assay time 30 min
Precision Average inter-assay CV: 7%Average intra-assay CV: 4.6%
Other instruments required Micropipettor, 37℃ incubator.
Other reagents required 60% Ethanol
Storage -20℃
Valid period 6 months

Images

TAS significantly decreased in diabetic model (DM), treatment of Cellgevity® significantly improved TAS. (**P<0.01, ***P<0.001)

O O Ogunlabi et al investigate the effects of Cellgevity® in diabetic model. Total antioxidants status (TAS) of rat serum was determined using TAS colorimetric assay kit (E-BC-K801-M).

Dilution of sample

It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.23-2 mmol Trolox Equiv. /L).

The recommended dilution factor for different samples is as follows (for reference only):

Sample type

Dilution factor

10% Mouse liver tissue homogenate

1

10% Rat liver tissue homogenate

1

10% Rat lung tissue homogenate

1

Molt4 cellar supernatant

1

Human urine

8-10

Mouse serum

1

Human serum

1

Human saliva

1

Note: The diluent is 60% Ethanol.

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Additional Information

Detection instrument:
Microplate reader(650 nm-670 nm,optimum wavelength: 660 nm)
Detection method:
Colorimetric method
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